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Merck
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重要文件

MRCF0R030

Millipore

Microcon®离心超滤管

NMWCO 30 kDa, Ultracel® regenerated cellulose membrane (low binding), sample volume 0.5 mL

同義詞:

Centrifuge concentrator

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About This Item

分類程式碼代碼:
41104916
eCl@ss:
36100101
NACRES:
NB.24

材料

Ultracel® regenerated cellulose membrane (low binding)
polyacetal base (membrane support)
polycarbonate device top
polypropylene tube (for filtrate/concentrate)
silicone O-ring (medical-grade)

品質等級

無菌

non-sterile

特點

holdup volume> 10 μL

製造商/商標名

Microcon®

參數

0.5 mL sample volume

技術

DNA purification: suitable
RNA purification: suitable
protein extraction: suitable
protein purification: suitable

長度

4.50 cm (1.8 in.)

直徑

12.3 mm

過濾面積

0.32 cm2

孔徑

30 kDa NMWCO

運輸包裝

ambient

一般說明

使用任何可以容纳1.5 mL离心管的离心机,简单而高效地对任何大分子溶液进行浓缩和除盐。借助低结合力的 Ultracel 滤膜,Microcon 过滤器可提供:
•具有 >95% 的典型回收率,甚至对稀释溶液也是如此
•能逆向旋转,最大限度地回收,甚至对最小的样品也是如此
•能非常方便地将滤出液或浓缩后样品储存在标准微型离心管中
•浓缩因子高达 100X

特征:
•逆转特性使得小体积样品也能达到高回收率(浓缩因子 <20X)
•低结合力 Ultracel 滤膜
•处理速度快

样品应用:
•法医应用中的基因组 DNA 回收
•核酸的浓缩和脱盐(是乙醇沉淀法的一种高回收率替代方法)
•从扩增的 DNA 中去除引物
•简便的滤液回收,得益于平整的滤膜设计

聯結

替代品:42410

法律資訊

MICROCON is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

10-13 - German Storage Class 10 to 13


分析證明 (COA)

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Flavio Oliveira et al.
Toxins, 12(3) (2020-04-05)
Toxic cyanobacterial blooms are a major contaminant in inland aquatic ecosystems. Furthermore, toxic blooms are carried downstream by rivers and waterways to estuarine and coastal ecosystems. Concerning marine and estuarine animal species, very little is known about how these species
Sarah K Stumper et al.
eLife, 8 (2019-02-06)
RNA polymerases (RNAPs) contain a conserved 'secondary channel' which binds regulatory factors that modulate transcription initiation. In Escherichia coli, the secondary channel factors (SCFs) GreB and DksA both repress ribosomal RNA (rRNA) transcription, but SCF loading and repression mechanisms are
Amrendra Mishra et al.
Carcinogenesis, 41(3), 334-344 (2019-06-07)
Beyond the nearly uniform presence of KRAS mutations, pancreatic cancer is increasingly recognized as a heterogeneous disease. Preclinical in vivo model systems exist, but with the advent of precision oncology, murine models with enhanced genetic flexibility are needed to functionally
Isaac A Klein et al.
Science (New York, N.Y.), 368(6497), 1386-1392 (2020-06-20)
The nucleus contains diverse phase-separated condensates that compartmentalize and concentrate biomolecules with distinct physicochemical properties. Here, we investigated whether condensates concentrate small-molecule cancer therapeutics such that their pharmacodynamic properties are altered. We found that antineoplastic drugs become concentrated in specific

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The use of PNGase Fast denaturing buffer and enzyme yielded results similar to a conventional 20-hour protocol with overnight digest while reducing workflow time to about 1 hour with a 15-minute digest.

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