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重要文件

MCVD2MAG-77K

Millipore

MILLIPLEX® Mouse Cardiovascular Disease (CVD) Magnetic Bead Panel 2 - Cardiovascular Disease Multiplex Assay

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About This Item

分類程式碼代碼:
12161503
eCl@ss:
32161000
NACRES:
NA.84

物種活性

mouse

品質等級

製造商/商標名

Milliplex®

assay range

accuracy: 84-106%
standard curve range: 137-100,000 pg/mL
(CD40L)

standard curve range: 14-10,000 pg/mL
(Endocan-1)

standard curve range: 274-200,000 pg/mL
(Follistatin)

standard curve range: 34-25,000 pg/mL
(CXCL16, Oncostatin M)

standard curve range: 4-3,000 pg/mL
(PIGF-2)

standard curve range: 686-500,000 pg/mL
(LIGHT, Troponin I)

standard curve range: 69-50,000 pg/mL
(Troponin T)

inter-assay cv: <20%
intra-assay cv: <10%

技術

multiplexing: suitable

檢測方法

fluorometric (Luminex xMAP)

運輸包裝

wet ice

一般說明

Cardiovascular disease (CVD), particularly atherosclerotic vascular disease, is a leading cause of global mortality, accounting for 30% of all global deaths (WHO). Inflammatory mechanisms play a vital role in the initiation, maintenance, and progression of vascular disease with a strong correlation between inflammatory markers and prognosis in acute and chronic coronary artery disease. Numerous studies have demonstrated an association of obesity and diabetes with cardiovascular risk
factors.

MILLIPLEX® Mouse CVD Panel 2 is a 9 plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma, or tissue culture samples: sCD40L, CXCL16, Endocan-1, Follistatin, LIGHT, Oncostatin M, PlGF-2, Troponin I and Troponin T. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Cardiovascular
The MILLIPLEX® MAP Mouse Cardiovascular Disease Panel 2 is used for the simultaneous quantification of any of the following analytes in serum, plasma, or culture supernatant samples: sCD40L, CXCL16, Endocan-1, Follistatin, LIGHT, Oncostatin M, PlGF-2, Troponin I, Troponin T.

特異性

Cross Reactivty
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.

應用

  • Analytes: sCD40L, CXCL16, Endocan-1 (ESM-1), Follistatin, LIGHT, Oncostatin M (OSM), , Placental Growth Factor 2 (PIGF-2), Troponin I (TnI), Troponin T (TnT)
  • Recommended Sample Type: Mouse serum, plasma, or cell/tissue culture supernatants or lysates
  • Recommended Sample Dilution: 10 μL per well of undiluted serum or plasma; cell/tissue culture supernatants may require dilution in appropriate control medium
  • Assay Run Time: Overnight (16-18 hours) at 2-8°C
  • Research Category: Cardiovascular Disease
  • Research Subcategory: Metabolism

特點和優勢

Design your multiplex kit by choosing available analytes within this panel.

包裝

Everything you need in a single kit.

成分

Mouse CVD Panel 2 Standard, 1 vial, lyophilized

Mouse CVD Panel 2 Quality Control 1, 1 vial, lyophilized

Mouse CVD Panel 2 Quality Control 2, 1 vial, lyophilized

Serum Matrix (0.08% Sodium Azide), 1 vial, lyophilized

Assay Buffer, 1 bottle, 30 mL

Bead Diluent, 1 bottle, 4 mL

Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea

Mouse CVD Panel 2 Detection Antibodies, 1 bottle, 5.5 mL

Streptavidin-Phycoerythrin, 1 bottle, 5.5 mL

Mixing Bottle, 1 bottle

Sample Plate, 96 well format, 1 plate

2 Foil Plate Sealers

儲存和穩定性

Recommended storage for kit components is 2 - 8°C.

其他說明

Please contact Technical Service for linearity of dilution.
Sensitivity: See kit protocol for individual analytes sensitivities.

法律資訊

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

象形圖

Skull and crossbonesEnvironment

訊號詞

Danger

危險分類

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects


分析證明 (COA)

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存取文件庫

David Sánchez-Infantes et al.
Obesity (Silver Spring, Md.), 25(1), 85-93 (2016-10-06)
Since oncostatin m (OSM) is elevated in adipose tissue in conditions of obesity and type 2 diabetes in mice and humans, the aim of this study was to determine whether this cytokine plays a crucial role in the impairment of

相關內容

Multiplex immunoassays, such as MILLIPLEX® multiplex metabolic assays, are critical in metabolic syndrome research because they provide a full picture of the different conditions related to it, like diabetes and obesity, as well as save time and sample volume.

我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.

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