MAC140

Anti-Inntag 10 (IT10) Antibody, clone IT10

clone IT10-1, from mouse

• 同義詞: Allergen Amb t V, Allergen Ra5G, Amb t 5
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: IT10-1, monoclonal
• 物種活性: Ambrosia trifida
• 物種活性（以同源性預測）: all
• 包裝: antibody small pack of 25 μg
• 技術: immunocytochemistry: suitable; western blot: suitable
• 同型: IgG1κ
• UniProt登錄號: P10414
• 運輸包裝: ambient
• 目標翻譯後修改: unmodified

描述

一般說明

Pollen allergen Amb t 5 (UniProt: P10414; also known as Allergen Amb t V, Allergen Ra5G, Amb t 5) binds to IgE and causes allergic reaction in human. Inntag IT10 is a small protein domain of Ambrosia trifida (Giant ragweed) that displays strong structural determinants and does not generate solubility issues in vivo and in vitro. Also, it does not compromise cell fitness when overexpressed, and has no detectable functional and localization effects when fused to a variety of target proteins. Structure of the Inntag IT10 is based on a packed beta-sheet conformation.

特異性

Clone IT10-1 detects pollen allergen Abt t 5 in HEK293 cells transfected with GFP-IT10.

免疫原

His-tagged recombinant peptide corresponding to 40 amino acids from Ambrosia trifida.

應用

Anti-Inntag 10 (IT10), clone IT10 is a mouse monoclonal antibody that detects the Innocuous Inntag IT10 based on pollen allergen Abt t5 sequence and is tested for use in Immunocytochemistry and Western Blotting.

Research Category
Secondary & Control Antibodies

Western Blotting Analysis: A representative lot detected Inntag 10 (IT10) in Western Blotting applications (Georgieva, M.V., et. al. (2015). Nat Methods. 12(10):955-8).

Immunocytochemistry Analysis: A representative lot detected Inntag 10 (IT10) in Immunocytochemistry applications (Georgieva, M.V., et. al. (2015). Nat Methods. 12(10):955-8).

品質

Evaluated by Western Blotting in transfected HEK293/GFP-IT10 cell lysates.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Inntag 10 (IT10) in 10 µg of transfected HEK293/GFP-IT10 cell lysates.

標靶描述

~33.7 kDa observed; 8.06 kDa calculated. 33.7 kD observed corresponds to 29.4 kDa GFP plus 4.3 kDa IT10. Uncharacterized bands may be observed in some lysate(s).

外觀

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG1 in PBS without azide.

儲存和穩定性

Stable for 1 year at -20°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable