MABT850M

Anti-Podoplanin Antibody, clone PMab-2

clone PMab-2, from mouse

• 同義詞: E11 antigen epitope, Glycoprotein 38, Gp38, OTS-8, RTI140, T1-alpha, T1A, Type I cell 40 kDa protein
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: PMab-2, monoclonal
• 物種活性: rat
• 包裝: antibody small pack of 25 μg
• 技術: ELISA: suitable; flow cytometry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• 同型: IgG1κ
• UniProt登錄號: Q64294
• 目標翻譯後修改: unmodified

描述

一般說明

Podoplanin (UniProt: Q64294; also known as E11 antigen epitope, Glycoprotein 38, Gp38, OTS-8, RTI140, T1-alpha, T1A, Type I cell 40 kDa protein) is encoded by the Pdpn (also known as Gp38, Ots8) gene (Gene ID: 54320) in rat. Podoplanin serves as the endogenous ligand of C-type lectin-like receptor-2 (CLEC-2) and is highly expressed in various tumors and in some normal cells. It may be involved in cell migration and/or actin cytoskeleton organization. Podoplanin is localized to actin-rich microvilli and plasma membrane projections, such as filopodia, lamellipodia, and ruffles. It is synthesized with a signal peptide of 22 amino acids, which is cleaved to produce the mature form. Podoplanin contains an extracellular domain (aa 23-135), a helical domain (aa 136-156) and a cytoplasmic region (aa 157-166). Podoplanin is extensively O-glycosylated and contains sialic acid residues. O-glycosylation is believed to be essential for its platelet aggregation activity. Clone PMab2 exhibits high affinity for podoplanin (Kd = 0.59 nM). (Ref.: Oki, H et al. (2015). Monoclon. Antib. Immunodiagn. Immunother. 34(6); 396-403).

特異性

Clone PMab-2 detects podoplanin in rat spleen and kidney. It targets an epitope within 14 amino acids from the N-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 14 amino acids from the N-terminal region of rat Podopalnin.

應用

Anti-Podoplanin, clone PMab-2, Cat. No. MABT850, is a mouse monoclonal that detects rat podoplanin and has been tested for use in ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), and Western Blotting.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Podoplanin in rat spleen tissue.

Flow Cytometry Analysis: A representative lot detected Podoplanin in Flow Cytometry applications (Oki, H., et. al. (2015). Monoclon Antib Immunodiagn Immunother. 34(6):396-403).

Western Blotting Analysis: A representative lot detected Podoplanin in Western Blotting applications (Oki, H., et. al. (2015). Monoclon Antib Immunodiagn Immunother. 34(6):396-403).

Immunohistochemistry Analysis: A representative lot detected Podoplanin in Immunohistochemistry applications (Oki, H., et. al. (2015). Monoclon Antib Immunodiagn Immunother. 34(6):396-403).

ELISA Analysis: A representative lot detected Podoplanin in ELISA applications (Oki, H., et. al. (2015). Monoclon Antib Immunodiagn Immunother. 34(6):396-403).

品質

Evaluated by Immunohistochemistry in rat kidney tissue sections.

Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected Podoplanin in rat kidney tissue sections.

標靶描述

17.58 kDa calculated.

外觀

Format: Purified

其他說明

Concentration: Please refer to lot specific datasheet.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1