MABT845

Anti-Cytokeratin-20 Antibody, clone XQ1

clone XQ1, from mouse

• 同義詞: Keratin, type I cytoskeletal 20, CK-20, Keratin-20, K20
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: XQ1, monoclonal
• 物種活性: mouse
• 應無反應活性: human
• 包裝: antibody small pack of 25 μg
• 技術: immunofluorescence: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• 同型: IgMκ
• NCBI登錄號: NP_075745
• UniProt登錄號: Q9D312
• 目標翻譯後修改: unmodified
• 基因資訊: human ... KRT20(54474) ; mouse ... Krt20(66809)

描述

一般說明

Keratin, type I cytoskeletal 20 (UniProt: Q9D312; also known as Cytokeratin-20, CK-20, Keratin-20, K20) is encoded by the Krt20 gene (Gene ID: 66809) in murine species. Cytokeratin-20 is type I keratin of the intermediate filament family and is recognized as a marker of intestinal differentiation. It is expressed at low levels in the more differentiated suprabasal regions of the small intestine, and at higher levels in the colon, mainly in the upper region and in scattered cells throughout the remaining epithelium. Cytokeratin-20 is also an excellent marker for certain types of carcinomas, such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. It plays a significant role in maintaining keratin filament organization in intestinal epithelia and when phosphorylated, it also plays a role in the secretion of mucin in the small intestine. Cytokeratin-20 can undergo hyperphosphorylation at serine 13 during the early stages of apoptosis, which becomes less prominent during the later stages. Phosphorylation at serine 13 is shown to increase in response to stress brought on by cell injury. During apoptosis it is cleaved at Asp 235 by caspases. (Ref.: Moll, R., et al. (1992). Am. J. Pathol. 140(2); 427-447).

特異性

Clone XQ1 is a mouse monoclonal antibody that detects cytoketatin-20 in murine tissues.

免疫原

A full-length purified murine Cytokeratin-20.

應用

Anti-Cytokeratin-20, clone XQ1, Cat. No. MABT845, is a mouse monoclonal antibody that detects Keratin, type I cytoskeletal 20 and has been tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.

Immunofluorescence Analysis: A representative lot detected Cytokeratin-20 in Immunofluorescence applications (Zhou, Q., et. al. (2003). Mol Biol Cell. 14(7):2959-71).

Western Blotting Analysis: A representative lot detected Cytokeratin-20 in Western Blotting applications (Zhou, Q., et. al. (2003). Mol Biol Cell. 14(7):2959-71).

Research Category
Cell Structure

品質

Evaluated by Immunohistochemistry (Paraffin) in mouse colon tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected Cytokeratin-20 in mouse colon tissue sections.

標靶描述

49.03 kDa calculated.

外觀

Format: Purified

Purified mouse monoclonal antibody IgM in PBS with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.