MABT1506

Anti-Desmoglein 3 Antibody, clone 5H10

clone 5H10, from mouse

• 同義詞: 130 kDa pemphigus vulgaris antigen, PVA, Cadherin family member 6
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: 5H10, monoclonal
• 物種活性: human
• 包裝: antibody small pack of 25 μg
• 技術: flow cytometry: suitable; immunocytochemistry: suitable; immunofluorescence: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• 同型: IgG1κ
• NCBI登錄號: NP_001935
• UniProt登錄號: P32926
• 目標翻譯後修改: unmodified
• 基因資訊: human ... DSG3(1830)

描述

一般說明

Desmoglein-3 (UniProt: P32926; also known as 130 kDa pemphigus vulgaris antigen, PVA, Cadherin family member 6) is encoded by the DSG3 (also known as CDHF6) gene (Gene ID: 1830) in human. Desmogleins are protein substances located in and on the surface of keratinocytes. They are present in specialized cell-cell adhesion structures enriched in tissues that undergo substantial mechanical stress. These proteins have been shown to be a critical factor in cell-to-cell adhesion. Desmoglein-3 is a single-pass type I membrane protein that is a component of intercellular desmosome junctions. It is involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. It is synthesized with a signal peptide (aa 1-23) and a propeptide (aa 24-49) that are cleaved off in the mature form. It contains an extracellular domain (aa 50-615), a transmembrane domain (aa 616-640), and a cytoplasmic domain (aa 641-999). Desmoglein-3 has four cadherin domains and two desmoglein repeats. This antibody (clone 5H10) recognized the extracellular domain of desmoglein-3.

特異性

Clone 5H10 detects Desmoglein-3 in human cells. It targets an epitope with in the extracellular domain.

免疫原

Epitope: extracellular domain

Extracellular domain of Desmoglein 3

應用

Anti-Desmoglein 3, clone 5H10, Cat. No. MABT1506, is a mouse monoclonal antibody that detects Desmoglein-3 and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Western Blotting.

Immunoprecipitation Analysis: A representative lot detected Desmoglein 3 in Immunoprecipitation applications (Wahl, J.K. 3rd, et. al. (2002). Hybrid Hybridomics. 21(1):37-44).

Flow Cytometry Analysis: A representative lot detected Desmoglein 3 in Flow Cytometry applications (Wan, H., et. al. (2007). Stem Cells. 25(5):1286-97).

Western Blotting Analysis: A representative lot detected Desmoglein 3 in Western Blotting applications (Wahl, J.K. 3rd, et. al. (2002). Hybrid Hybridomics. 21(1):37-44).

Immunocytochemistry Analysis: A representative lot detected Desmoglein 3 in Immunocytochemistry applications (Wahl, J.K. 3rd, et. al. (2002). Hybrid Hybridomics. 21(1):37-44).

Immunofluorescence Analysis: A representative lot detected Desmoglein 3 in Immunofluorescnece applications (Wan, H., et. al. (2007). Stem Cells. 25(5):1286-97).

Research Category
Cell Structure

品質

Evaluated by Western Blotting in HaCaT cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Desmoglein 3 in HaCaT cell lysate.

標靶描述

~125 kDa observed; 107.53 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外觀

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.