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重要文件

MABT121

Sigma-Aldrich

Anti-Vimentin Antibody, clone 3CB2

clone 3CB2, from mouse

同義詞:

Vimentin

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41
無性繁殖:
3CB2, monoclonal
application:
ICC
IHC
WB
物種活性:
chameleon (GLIA 15:389-400 (1995), Prada, F. A., et. al.), rat (GLIA 15:389-400 (1995), Prada, F. A., et. al.), human, lizard
技術:
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
citations:
3

生物源

mouse

品質等級

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

3CB2, monoclonal

物種活性

chameleon (GLIA 15:389-400 (1995), Prada, F. A., et. al.), rat (GLIA 15:389-400 (1995), Prada, F. A., et. al.), human, lizard

物種活性(以同源性預測)

chicken (immunogen homology)

技術

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

同型

IgMκ

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... VIM(7431)

一般說明

Vimentin is a class III intermediate filament protein (homopolymers) found in various non-epithelial cells including mesenchymal cells and fibroblasts. In many cells of mesenchymal origin, it is one of the most prominent phosphoproteins, especially during cell division when enhanced phosphorylation of vimentin corresponds with the reorganization of vimentin filaments.

免疫原

Vimentin extracted from chicken embryonic brain tissue.

應用

Anti-Vimentin Antibody, clone 3CB2 is an antibody against Vimentin for use in WB, IC, IH.
Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in human central and peripheral retinal sections (Perez-Alvarez, M.J., et al. (2008). J Neurosci Res. 86(8):1871-1883).

Western Blot Analysis:
A representative lot was used to detect vimentin in chicken and rat total brain extracts (Prada, FA., et al. (1995). GLIA 15:389-400).

Immunohistochemistry Analysis:
A representative lot was used to detect vimentin in E3 chick embryo sections and different regions of the developing chick central nervous system (Prada, FA., et al. (1995). GLIA 15:389-400).
Research Category
Cell Structure
Research Sub Category
Cytoskeleton

品質

Evaluated by Western Blot in ARPE19 cell lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected Vimentin in ARPE19 cell lysate.

標靶描述

~54 kDa observed

外觀

Format: Purified
Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

分析報告

Control
ARPE cell lysate

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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Hong Qi et al.
Experimental and therapeutic medicine, 15(4), 3509-3515 (2018-03-17)
The present study aimed to investigate the effects of JWA knockout (JWA-/-) on malignant transformation of murine embryonic fibroblast (MEF) cells using a conditional JWA-/- mouse model. Once MEF cells were prepared, the potential role of JWA-/- on proliferation, migration
Yi Sui et al.
British journal of cancer, 125(7), 1003-1015 (2021-07-12)
Metastasis is the major cause of treatment failure and cancer-related deaths in prostate cancer (PCa) patients. Our previous study demonstrated that a CD44+ subpopulation isolated from PCa cells or tumours possesses both stem cell properties and metastatic potential, serving as

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