MABT12

Anti-Heparan Sulfate Proteoglycan (Perlecan) Antibody, clone 5D7-2E4

clone 5D7-2E4, from mouse

• 同義詞: heparan sulfate proteoglycan 2 (domain V region), perlecan proteoglycan, basement membrane-specific heparan sulfate proteoglycan core protein, HSPG, PLC
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: 5D7-2E4, monoclonal
• 物種活性: human
• 技術: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• 同型: IgG1κ
• NCBI登錄號: NP_005520
• UniProt登錄號: P98160
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... HSPG2(3339)

描述

一般說明

Perlecan is one of the extracellular matrix forms of heparan sulfate proteoglycans (HSPGs). It is a large multidomain and binds to and cross-links many extracellular matrix (ECM) components and cell-surface molecules. Perlecan is synthesized by vascular endothelial and smooth muscle cells and deposited in the extracellular matrix within all mammalian tissues. HSPGs differ in their protein cores that are thought to determine the location of HSPG in either the cell membrane (syndecan and glypican) or the extracellular matrix (perlecan and dystroglycan). HSPGs found in the brain are dystroglycan, N-syndecan glypican, and perlecan. The composition of HS isolated from brain differs significantly from that of other organs and is developmentally regulated. Thus, differences in protein cores, disaccharide composition, the extent and position of sulfation, and the number of GAG chains diversify chemical structure and functions of HSPGs. The postulated functions of HSPG include cell proliferation, differentiation, adhesion, migration, and morphogenesis.

免疫原

Full length recombinant protein corresponding to human Perlecan.

應用

Anti-Heparan Sulfate Proteoglycan (Perlecan) Antibody, clone 5D7-2E4 detects level of Heparan Sulfate Proteoglycan (Perlecan) & has been published & validated for use in IH.

Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC. (courtesy of Whitelock, J. Graduate School of Biomedical Engineering, University of New South Wales, Sydney, Australia)

Western Blot Analysis: A previous lot was used by an independent laboratory on a 3-8% Tris-acetate gel in which the band appears above the 460 kDa marker. (courtesy of Whitelock, J. Graduate School of Biomedical Engineering, University of New South Wales, Sydney, Australia)

Research Category
Cell Structure

Research Sub Category
ECM Proteins

品質

Evaluated by Immunohistochemistry in fresh-frozen normal human tonsil tissue.

Immunohistochemistry Analysis: 1:300 dilution of the antibody detected Perlecan in fresh-frozen normal human tonsil tissue.

標靶描述

469 kDa calculated

外觀

Protein G

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

分析報告

Control
Fresh-frozen normal human tonsil tissue

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable