MABS461

Anti-Neutrophil Elastase Antibody, clone AHN-10

clone AHN-10, from mouse

• 同義詞: Neutrophil elastase, Bone marrow serine protease, Elastase-2, Human leukocyte elastase, HLE, Medullasin, PMN elastase
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: AHN-10, monoclonal
• 物種活性: human
• 技術: immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; inhibition assay: suitable; radioimmunoassay: suitable
• 同型: IgG1κ
• NCBI登錄號: NP_001963.1
• UniProt登錄號: P08246
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... ELANE(1991)

描述

一般說明

Neutrophil Elastase, also known as Bone marrow serine protease, Elastase-2, Human leukocyte elastase (HLE), Medullasin or PMN elastase, and encoded by the gene ELANE/ELA2, is a serine proteinase in the same family as chymotrypsin. Neutrophil Elastase has broad substrate specificity and is part of our host defense mechanism against bacteria. Unfortunately passively released or actively secreted elastase from neutrophils has been linked to the pathologic processes of a variety of inflammatory diseases, including idiopathic pulmonary fibrosis, rheumatoid arthritis, adult respiratory distress syndrome, and cystic fibrosis. Neutrophil Elastase is localized to the cytoplasm in granules. Neutrophil Elastase is expressed in neutrophils and macrophages. Defects in Neutrophil Elastase may be associated with cyclic neutropenia and congential neutropenia diseases where neutrophils fail to mature leaving the patient very vunderable to infections.

特異性

This antibody detects with human Neutrophil Elastase but not other major neutrophil proteases: Cathapsin G, proteinase 3, collagenase, or esterase N (Skubitz, K. M., et al. (1988). J Leukoc Biol. 44(3):158-165.).

免疫原

Whole cells corresponding to human Neutrophil Elastase.

應用

Detect Elastase using this mouse monoclonal antibody, Anti-Neutrophil Elastase Antibody, clone AHN-10 validated for use in ICC, IHC, IP, Radioimmunoassay & Inhibition.

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Neutrophil Elastase in unirradiated and irradiated human and mouse skin tissues (Rhodes, L. E., et al. (2009). FASEB J. 23(11): 3947-3956.; Nicolaou, A., et al. (2012). Photochem Photobiol Sci. 11(2):371-380.; Rutter, J. J., et al. (2009). 129(12):2861-2867.).

Western Blotting Analysis: A representative lot from an independent laboratory detected Neutrophil Elastase in human pancreatic tissue lysate (Mayerle, J., et al. (2005). Gastroenterology. 129(4):1251-1267.).

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Neutrophil Elastase in human pancreatic tissue lysate (Mayerle, J., et al. (2005). Gastroenterology. 129(4):1251-1267.).

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Neutrophil Elastase in human acinar cells (Mayerle, J., et al. (2005). Gastroenterology. 129(4):1251-1267.).

Inhibition Analysis: This assay inhibits the elastinolytic actiity of purified human Neutrophil Elastase (Skubitz, K., et al. (1988). J Leukoc Biol. 44(3):158-165.).

Radioimmunoassay Analysis: A representative lot from an independent laboratory detected Neutrophil Elastase using purified Neutrophil Elastase (Skubitz, K., et al. (1988). J Leukoc Biol. 44(3):158-165.).

Research Category
Cell Structure

Research Sub Category
ECM Proteins

品質

Evaluated by Immunocytochemistry Analysis in peripheral blood mononuclear cells.

Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected Neutrophil Elastase in peripheral blood mononuclear cells.

標靶描述

28 kDa calculated

外觀

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable