MABS460-I

Anti-FGF23 Antibody, clone 278-2

• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: human
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 278-2, monoclonal
• 分子量: calculated mol wt 27.95 kDa; observed mol wt ~37 kDa
• 純化經由: using protein G
• 物種活性: human
• 包裝: antibody small pack of 100 μg
• 技術: flow cytometry: suitable; western blot: suitable
• 同型: IgG1κ
• 表位序列: Unknown
• Protein ID登錄號: NP_065689
• UniProt登錄號: Q9GZV9
• 運輸包裝: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... fgf23> FGF23(8074)

描述

一般說明

Fibroblast growth factor 23 (UniProt: Q9GZV9; also known as FGF-23, Phosphatonin, Tumor-derived hypophosphatemia-inducing factor) is encoded by the FGF23 (also known as HYPF, UNQ3027/PRO9828) gene (Gene ID: 8074) in human. FGF23 is a growth factor that is produced primarily in the bone tissue and its primary hormonal function is to regulate phosphate homeostasis and vitamin D metabolism. Unlike other FGFs, it has a low affinity for heparin sulfate, which allows it to enter the circulation and act as an endocrine hormone. It inhibits renal tubular phosphate transport by reducing sodium-dependent phosphate transport protein 2A (SLC34A1) levels. FGF23 binding to FGFR requires a co‐receptor, Klotho, which acts as a scaffold for docking with FGFR1. Together with Klotho, it modulates phosphate reabsorption and both 1 ‐hydroxylation and 24‐hydroxylation in the renal proximal tubules. FGF23 is reported to inhibit the expression of 1 ‐hydroxylase while increasing the expression of 24‐hydroxylase, thus reducing the concentration of active 1,25(OH)2D. It can also act directly on the parathyroid to reduce parathyroid hormone secretion. (Ref.: Almel, EA., et al. (2019). J. Bone Min. Res (JMBR Plus). 3(8): e10190).

特異性

Clone 278.2 is a mouse monoclonal antibody that detects human Fibroblast growth factor 23 (FGF-23).

免疫原

Recombinant human Fibroblast growth factor 23 (FGF23).

應用

Quality Control Testing

Evaluated by Western Blotting in Human brain tissue lysates.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected FGF23 in Human brain tissue lysates.

Tested Applications:

Flow Cytometry Analysis (FC): 1 μg of this antibody detected Fibroblast growth factor 23 (FGF23) in one million Jurkat cells pretreated with Brefeldin A (1 μg/mL 4 h).

Anti-FGF23, clone 278-2, Cat. No. MABS460-I, is a mouse monoclonal antibody that detects Fibroblast growth factor 23 and is tested for use in Flow Cytometry and Western Blotting.

外觀

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable