推薦產品
生物源
mouse
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
4F1, monoclonal
物種活性
rat, mouse, human
技術
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同型
IgG2a
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... UBD(10537)
一般說明
FAT10 protein is a recombinant protein produced in E. coli corresponding to the human sequence encompassing an N-terminal His6-tag within a leader sequence. FAT10, a ubiquitin-like protein, is encoded in the major histocompatibility complex class I locus and is synergistically inducible with interferon-gamma and tumor necrosis factor alpha. FAT10 expression causes apoptosis and it appears that FAT10 may modulate tumorigenesis through its interaction with the MAD2 spindle-assembly checkpoint protein. It has been demonstrated that FAT10 and its conjugates are rapidly degraded by the proteasome. Furthermore, a new interaction partner of FAT10, NEDD8 ultimate buster-1L (NUB1L), may function as a linker that targets FAT10 for degradation by the proteasome.
免疫原
GST-tagged recombinant protein corresponding to human FAT10.
應用
Anti-FAT10 Antibody, clone 4F1 is a highly specific mouse monoclonal antibody, that targets FAT10 & has been tested in western blotting, IP & ICC.
Immunocytochemistry Analysis: A representative lot detected FAT10 in HA-FAT10 transfected HEK293 cells (Aichem, A., et al. (2010). Nature Commun. 1:13).
Immunoprecipitation Analysis: A representative lot immunoprecipitated FAT10 in transfected HA-FAT10 HEK293 cells cultivated with IFNgamma and TNFalpha (Aichem, A., et al. (2010). Nature Commun. 1:13).
Immunoprecipitation Analysis: A representative lot immunoprecipitated FAT10 in transfected HA-FAT10 HEK293 cells cultivated with IFNgamma and TNFalpha (Aichem, A., et al. (2010). Nature Commun. 1:13).
品質
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected FAT10 in 10 µg of HepG2 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected FAT10 in 10 µg of HepG2 cell lysate.
標靶描述
~18 kDa observed. FAT10 FAT10ylates itself as well as other proteins so additional bands may be observed due to the FAT10ylation of endogenous proteins (Aichem, A., et al. (2010). Nature Commun. 1:13.),(Aichem, A., et al. J Cell Sci. (2012)125:4576-85).
外觀
Format: Purified
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Annette Aichem et al.
Journal of cell science, 125(Pt 19), 4576-4585 (2012-07-17)
FAT10 is a ubiquitin-like modifier proposed to function in apoptosis induction, cell cycle control and NF-κB activation. Upon induction by pro-inflammatory cytokines, hundreds of endogenous substrates become covalently conjugated to FAT10 leading to their proteasomal degradation. Nevertheless, only three substrates
Annette Aichem et al.
Nature communications, 1, 13-13 (2010-10-27)
The ubiquitin-like modifier FAT10 targets proteins for degradation by the proteasome and is activated by the E1 enzyme UBA6. In this study, we identify the UBA6-specific E2 enzyme (USE1) as an interaction partner of FAT10. Activated FAT10 can be transferred
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