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Key Documents

MABS270

Sigma-Aldrich

Anti-PP2A reg. subunit B56 epsilon (PPP2R5E) Antibody, clone 5A5-1F3

clone 5A5-1F3, from mouse

同義詞:

Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilon isoform, PP2A B subunit isoform B′-epsilon,

PP2A B subunit isoform B56-epsilon, PP2A B subunit isoform PR61-epsilon, PP2A B subunit isoform R5-epsilon

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

mouse

品質等級

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

5A5-1F3, monoclonal

物種活性

human, mouse

技術

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

同型

IgG1κ

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... PTPA(5524)

一般說明

Protein phosphatase 2A (PP2A) is a family of ubiquitous heterotrimeric proteins that catalyze the removal of phosphate groups from serine and threonine residues. The PP2A enzymes contain core structural (A) and catalytic (C) subunits and variable regulatory B subunits. The B subunits regulate their catalytic activity, as well as dictate substrate specificity and subcellular localization. Recent studies have suggested that B56 epsilon subunits regulate p53-mediated apoptotic pathways, and can induce or suppress apoptosis depending on the cellular context. PP2A enzymes are also regulated by posttranslational modifications and endogenous inhibitors.

特異性

This antibody is specific for the B56 regulatory subunit of Protein phosphatase 2A (PP2A).

免疫原

His-tagged recombinant protein corresponding to human PP2A reg. subunit B56 epsilon (PPP2R5E).

應用

Research Category
Neuroscience

Apoptosis & Cancer
Research Sub Category
Kinases & Phosphatases

Apoptosis - Additional
This Anti-PP2A reg. subunit B56 epsilon (PPP2R5E) Antibody, clone 5A5-1F3 is validated for use in Western Blotting, ICC, Immunoprecipitation for the detection of PP2A reg. subunit B56 epsilon (PPP2R5E).
Western Blot Analysis: A 1:1,000 dilution from a representative lot detected PP2A reg. subunit B56 epsilon (PPP2R5E) in 10 µg of U2OS cell lysate and mouse brain tissue lysate.

Immunocytochemistry Analysis: A representative lot detected PP2A reg. subunit B56 epsilon (PPP2R5E) in U2OS cells. (Max F. Perutz Laboratories, Medical University of Vienna, Austria).

Immunoprecipitation Analysis: A representative lot immunoprecipitated endogenous PP2A reg. subunit B56 epsilon (PPP2R5E) in a panel of U2OS cell lysates expressing the B56 alpha, beta, gamma, delta, and epsilon regulatory subunit of PP2A (Max F. Perutz Laboratories, Medical University of Vienna, Austria).

品質

Evaluted by Western Blot in NIH/3T3 cell lysate.

Western Blot Analysis: A 1:1,000 dilution from a representative lot detected PP2A reg. subunit B56 epsilon (PPP2R5E) in 10 µg of NIH/3T3 cell lysate.

標靶描述

~50 kDa observed

外觀

Protein G Purified
Format: Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

分析報告

Control
NIH/3T3 cell lysate

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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Ulrich Baumgartner et al.
Molecular cancer, 17(1), 44-44 (2018-02-20)
Epidermal growth factor receptor (EGFR) mutations enable constitutive active downstream signaling of PI3K/AKT, KRAS/ERK and JAK/STAT pathways, and promote tumor progression by inducing uncontrolled proliferation, evasion of apoptosis and migration of non-small cell lung cancer (NSCLC). In addition, such EGFR
Huan He et al.
eLife, 7 (2018-10-16)
The intracellular parasite Toxoplasma gondii, hijacks evolutionarily conserved host processes by delivering effector proteins into the host cell that shift gene expression in a timely fashion. We identified a parasite dense granule protein as GRA18 that once released in the
Qing Kang et al.
The Journal of biological chemistry, 289(25), 17480-17496 (2014-05-16)
The mitotic kinase Aurora A (AurA) is regulated by a complex network of factors that includes co-activator binding, autophosphorylation, and dephosphorylation. Dephosphorylation of AurA by PP2A (human, Ser-51; Xenopus, Ser-53) destabilizes the protein, whereas mitotic dephosphorylation of its T-loop (human
Jade J Dyson et al.
FASEB bioAdvances, 4(4), 273-282 (2022-04-14)
Protein phosphatase 2A (PP2A) is a heterotrimeric phosphatase that controls a wide range of cellular functions. The catalytic activity and intracellular location of PP2A are modulated by its association with regulatory B subunits, including B56 proteins, which are encoded by

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