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Merck
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重要文件

MABS2317

Sigma-Aldrich

Anti-SIRPA/CD172a Antibody, clone SE12C3

同義詞:

Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A, CD172a, Inhibitory receptor SHPS-1, Macrophage fusion receptor MyD-1 antigen, SHP substrate 1, SHPS-1, Signal-regulatory protein alpha-1, Signal-regulatory protein alpha-2, Signal-regulatory protein alpha-3, Sirp-alpha-1, Sirp-alpha-2, Sirp-alpha-3, Tyrosine-protein phosphatase non-receptor type substrate 1, p84

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

mouse

品質等級

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

SE12C3, monoclonal

分子量

calculated mol wt 55 kDa
observed mol wt ~N/A kDa

純化經由

using protein G

物種活性

human

包裝

antibody small pack of 100

技術

ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
inhibition assay: suitable

同型

IgG1

表位序列

Extracellular domain

Protein ID登錄號

UniProt登錄號

儲存溫度

-10 to -25°C

基因資訊

human ... SIRPA(140885)

特異性

Clone SE12C3 is a mouse monoclonal antibody that detects SIRPA/CD172a. It targets an epitope within the extracellular domain.

免疫原

GST-tagged recombinant extracellular domain of human SIRPA/CD172a.

應用

Quality Control Testing

Evaluated by Flow Cytometry in Human peripheral blood mononuclear cells (PBMC).

Flow Cytometry Analysis: 1.0 µg of this antibody detected SIRPA/CD172a in one million Human peripheral blood mononuclear cells (PBMC).

Tested Applications

Immunoprecipitation Analysis: A representative lot immunoprecipitated SIRPA/CD172a in Immunoprecipitation applications (Seiffert, M., et al. (1999). Blood. 94(11):3633-43).

Inhibition Assay: A representative lot inhibited the binding of soluble SIRPA to cells. (Seiffert, M., et al. (1999). Blood. 94(11):3633-43; Murata, Y., et al. (2018). Cancer Sci. 109(5):1300-1308).

Immunocytochemistry Analysis: A representative lot detected SIRPA/CD172a in Immunocytochemistry applications (Seiffert, M., et al. (1999). Blood. 94(11):3633-43; Murata, Y., et al. (2018). Cancer Sci. 109(5):1300-1308).

ELISA Analysis: A representative lot detected SIRPA/CD172a in ELISA applications (Murata, Y., et al. (2018). Cancer Sci. 109(5):1300-1308).

Flow Cytometry Analysis: A representative lot detected SIRPA/CD172a in Flow Cytometry applications (Seiffert, M., et al. (1999). Blood. 94(11):3633-43).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

標靶描述

Tyrosine-protein phosphatase non-receptor type substrate 1 (UniProt: P78324; also known as SHP substrate 1, SHPS-1, Brain Ig-like molecule with tyrosine-based activation motifs, Bit, CD172 antigen-like family member A, Inhibitory receptor SHPS-1, Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory protein alpha-1, Sirp-alpha-1, Signal-regulatory protein alpha-2, Sirp-alpha-2, Signal-regulatory protein alpha-3, Sirp-alpha-3, p84, CD172a) is encoded by the SIRPA (also known as BIT, MFR, MYD1, PTPNS1, SHPS1, SIRP) gene (Gene ID: 140885) in human. SIRPA/CD172a is a single-pass type I membrane protein of the Ig superfamily that is ubiquitously expressed with high expression reported in brain. It is also detected on myeloid cells, but not on T cells. SIRPA is synthesized with a signal peptide (aa 1-30), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 31-373), a transmembrane domain (aa 374-394) and a cytoplasmic domain (aa 395-504). The extracellular region contains three Ig-like loops. The most distal loop (D1) contains and Ig-like V type domain (aa 32-137) whereas the two membrane proximal loops contain Ig-like C1 type domains (aa 148-247 and 254-348). SIRPA serves as an immunoglobulin-like cell surface receptor for CD47, and their interactions is reported to mediate negative regulation of several monocyte/ macrophage functions. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. SIRP alpha also acts as a docking protein and induces translocation of PTPN6, PTPN11, and other binding partners from the cytosol to the plasma membrane. SIRPA is phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin, and platelet derived growth factor. (Ref.: Murata, Y., et al., ( 2018). Cancer Sci. 109(5); 1300-1308; Lee, WY et al., (2007). J. Immunol.; 179(11); 7741-7750; Seiffert, M., et al (1999). Blood. 94(11); 3633-3643).

外觀

Purified mouse monoclonal antibody IgG1 in PBS without preservatives.

重構

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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