MABS2309

Anti-p66shc Antibody, clone BG8

• 同義詞: SHC-transforming protein 1;SHC-transforming protein 3;SHC-transforming protein A;Src homology 2 domain-containing-transforming protein C1;SH2 domain protein C1
• 分類程式碼代碼: 12352203

屬性

• 生物源: mouse
• 品質等級: 200
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: BG8, monoclonal
• 分子量: calculated mol wt 62.82 kDa; observed mol wt ~62 kDa
• 純化經由: using protein G
• 物種活性: mouse
• 物種活性（以同源性預測）: human
• 包裝: antibody small pack of 100 μL
• 技術: immunocytochemistry: suitable; immunofluorescence: suitable; western blot: suitable
• 同型: IgG1κ
• 表位序列: N-terminal
• Protein ID登錄號: NP_001123512
• UniProt登錄號: P29353
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... SHC1(6464)

描述

一般說明

SHC-transforming protein 1 (UniProt: P29353; also known as SHC-transforming protein 3, SHC-transforming protein A, Src homology 2 domain-containing-transforming protein C1, SH2 domain protein C1) is encoded by the SHC1 (also known as SHC, SHCA) gene (Gene ID: 6464) in human. p66Shc is a splice variant of p52Shc/p46Shc with a typical domain organization that is shared by all members of the Shc family of adaptor proteins. It has a phosphotyrosine binding domain, a collagen homology 1 region, and an SH2 domain. p66Shc is shown to be a downstream target of p53 and activated p53 induces its up-regulation by increasing its stability. p66Shc is suggested to be a genetic determinant of aging in mammals as its homozygous deletion is reported to delay senescence and prolong life span in mice. It regulates the mitochondrial pathway of apoptosis by inducing mitochondrial damage after dissociation from an inhibitory protein complex. Under conditions of excessive reactive oxygen species (ROS), p66Shc is phosphorylated on serine 36 by protein kinase C and is targeted to the mitochondrial intermembrane space where it serves as a redox enzyme by oxidizing cytochrome c and reducing oxygen to form ROS. In the absence of p66Shc, release of cytochrome c from mitochondria is impaired and apoptosome can only be activated if purified cytochrome c is supplied. p66Shc induces the collapse of the mitochondrial trans-membrane potential after oxidative stress. p66Shc is also shown to have a neuroprotective role in a murine model of multiple sclerosis. p66Shc knockout (p66-KO) mice develop typical signs of experimental autoimmune encephalomyelitis (EAE). (Ref.: Su, KG., et al. (2012). Eur. J. Neurosci. 35; 562-571; Orsini, F., et al. (2004). J. Biol. Chem. 279(24); 25689-25695).

特異性

Clone BG8 is a mouse monoclonal antibody that detects p66Shc. It was generated in p66Shc-/- mice. It targets an epitope within the N-terminal region.

免疫原

GST-tagged recombinant fragment corresponding to the first 110 amino acids from the N-terminal region of human SHC-transforming protein 1 (p66Shc).

應用

Quality Control Testing

Evaluated by Western Blotting in NIH3T3 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected SHC-transforming protein 1 in NIH3T3 cell lysate.

Tested Applications

Immunofluorescence Analysis: A representative lot detected p66shc 1 in Immunofluorescence application (Orsini, F., et al. (2004). J Biol Chem. 279(24): 25689-95).

Immunocytochemistry Analysis: A representative lot detected p66shc in Immunocytochemistry application (Orsini, F., et al. (2004). J Biol Chem. 279(24): 25689-95).

Western Blotting Analysis: A representative lot detected p66shc in Western Blotting application (Orsini, F., et al. (2004). J Biol Chem. 279(24): 25689-95); Su, K.G., et al. (2012). Eur J Neurosci. 35(4):562-71).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

外觀

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

重構

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommended storage: +2°C to +8°C.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1