MABS2291

Anti-DPP4/CD26 Antibody, clone KU44.13A

• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: KU44.13A, monoclonal
• 分子量: calculated mol wt 88.28 kDa
• 純化經由: using protein G
• 物種活性: human
• 包裝: antibody small pack of 100 μL
• 技術: ELISA: suitable; flow cytometry: suitable; immunofluorescence: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunoprecipitation (IP): suitable
• 同型: IgG2κ
• 表位序列: Extracellular domain
• Protein ID登錄號: NP_001926
• UniProt登錄號: P27487
• 運輸包裝: 2-8°C
• 目標翻譯後修改: unmodified

描述

一般說明

Dipeptidyl peptidase 4 (UniProt: P27487; also known as EC:3.4.14.5, ADABP, Adenosine deaminase complexing protein 2, ADCP-2, Dipeptidyl peptidase IV, DPP IV, T-cell activation antigen CD26, TP103, CD26) is encoded by the DPP4 (also known as CD26, ADCP2) gene (Gene ID: 1803) in human. DPP4 is a single-pass type II membrane glycoprotein that is ubiquitously expressed on the surface of a variety of cells. It is expressed in endothelial cells, fibroblasts, and lymphocytes and on the apical surfaces of epithelial and acinar cells. It serves as a serine exopeptidase that cleaves X-proline or X-alanine dipeptides from the N-terminus of polypeptides. A soluble form of DPP4 is also present (aa 39-766) that is derived from the membrane by proteolytic processing. DPP4 expression is substantially dysregulated in a variety of disease states including inflammation, cancer, obesity, and diabetes and its inhibition by the gliptin family of drugs has gained importance for the therapy of type 2 diabetes. DPP4 is also involved in the costimulatory signal that is essential for T-cell receptor (TCR)-mediated T-cell activation. Its levels are up-regulated by IL-12 on activated T-cells and down-regulated by TNF. DPP4 also plays a role in tumor development and its effect depends on the tumor type and its microenvironment. It has a tumor suppressor effect in several cancers including melanoma, ovarian cancer, non-small cell lung cancer, prostate cancer, endometrial cancer, and neuroblastoma. Higher levels of DPP4 have been observed in pancreatic cancer and knockdown of DPP4 expression inhibits cell growth, migration, and invasion. (Ref.: Arias-Pinilla, GA., et al. (2020). Sci. Rep. 10; 537; Röhrborn, D., et al. (2015). Front. Immunol. 6; 386).

特異性

Clone KU44.13A is a mouse monoclonal antibody that detects Dipeptidyl peptidase 4 (DPP4)/CD26.

免疫原

CFPAC-1 human pancreatic cancer cells and SP2 myeloma cells.

應用

Quality Control Testing

Evaluated by Flow Cytometry in HPAF-II cells.

Flow Cytometry Analysis: 1 μg of this antibody detected DPP4/CD26 in one million HPAF-II cells.

Tested Applications

Immunofluorescence Analysis: A representative lot detected DPP4/CD26 in Immunofluorescence applications (Arias-Pinilla, G.A., et al. (2020). Sci Rep. 10(1):537).

Immunoprecipitation Analysis: A representative lot immunoprecipitated DPP4/CD26 in Immunoprecipitation applications (Arias-Pinilla, G.A., et al. (2020). Sci Rep.10(1):537).

ELISA Analysis: A representative lot detected DPP4/CD26 in ELISA applications (Arias-Pinilla, G.A., et al. (2020). Sci Rep. 10(1):537).

Flow Cytometry Analysis: A representative lot detected DPP4/CD26 in Flow Cytometry applications (Arias-Pinilla, G.A., et al. (2020). Sci Rep. 10(1):537).

Immunohistochemistry Applications: A representative lot detected DPP4/CD26 in Immunohistochemistry applications (Arias-Pinilla, G.A., et al. (2020). Sci Rep. 10(1):537).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

This Anti-DPP4/CD26, clone KU44.13A, Cat. No. MABS2291, is a mouse monoclonal antibody that detects DPP4/CD26 and is tested for use in ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, and Immunoprecipitation.

外觀

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable