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重要文件

MABS2273

Sigma-Aldrich

Anti-CD200/HPi1 Antibody, clone HIC0-4F9

同義詞:

OX-2 membrane glycoprotein

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.43

生物源

mouse

品質等級

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

HIC0-4F9, monoclonal

分子量

calculated mol wt 31.26 kDa
observed mol wt ~N/A kDa

純化經由

using protein G

物種活性

human

包裝

antibody small pack of 100

技術

flow cytometry: suitable
immunohistochemistry: suitable

同型

IgG1κ

表位序列

Unknown

Protein ID登錄號

UniProt登錄號

儲存溫度

2-8°C

基因資訊

human ... CD200(4345)

特異性

Clone HIC0-4F9 is a mouse monoclonal antibody that detects CD200/HPi1 protein.

免疫原

Human pancreatic enriched islet cells.

應用

Quality Control Testing

Isotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1 .

Tested Applications

Immunohistochemistry Applications: A representative lot detected CD200/HPi1 in Immunohistochemistry applications in fresh-frozen normal pancreas tissues (Courtesy of Craig Dorrell, Ph.D., Oregon Health & Science University, Portland, Oregon USA).

Immunohistochemistry Applications: A representative lot detected CD200/HPi1 in Immunohistochemistry applications (Dorrell, C., et al. (2008). Stem Cell Res. 1(3):183-94).

Flow Cytometry Analysis: A representative lot detected CD200/HPi1 in Flow Cytometry applications (van der Heide, V., et al. (2022). Cell Rep. 38(11):110508).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

標靶描述

OX-2 membrane glycoprotein (UniProt: P41217; also known as CD200) is encoded by the CD200 (also known as MOX1, MOX2, My033) gene (Gene ID: 4345) in human. CD200 is a widely expressed, single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-30), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 31-232), a transmembrane domain (aa 233-359), and a cytoplasmic domain (aa 260-278). It also contains one V-type Ig-like domain (aa 31-141) and one C2-type Ig-like domain (aa 142-232). It is widely expressed on a broad range of cells including lymphoid cells, neurons, and endothelium. It interacts with CD200R, an immunoglobulin superfamily inhibitory receptor that is expressed primarily on myeloid/monocyte lineage cells and has a suppressive effect on T cell-mediated immune response. CD200 also serves as a pani-slet marker of pancreatic endocrine cells, including insulin-expressing β- cells, but does not recognize exocrine cells. Its levels are reported to be elevated in pancreatic β cells following SARS-CoV-2 infection and this infection is strictly dependent on ACE2 receptor. Three isoforms of CD200 have been described that are produced by alternative splicing. (Ref.: van der Heide, V., et al. (2022). Cell Rep. 38(11); 110508; Dorfman, DM., and Shahsafaei, A. (2010). Am. J. Clin. Pathol. 134(5); 726-733; Dorrell, C., et al. (2008). Stem Cell Res. 1(3); 183-194).

外觀

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

重構

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommended storage: +2°C to +8°C.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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