MABS1905

Anti-TMEPAI Antibody, clone 9F10

clone 9F10, from mouse

• 同義詞: Prostate transmembrane protein androgen induced 1, Solid tumor-associated 1 protein, Transmembrane prostate androgen-induced protein
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: 9F10, monoclonal
• 物種活性: human, monkey
• 技術: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• 同型: IgG1κ
• NCBI登錄號: NP_064567
• UniProt登錄號: Q969W9
• 運輸包裝: ambient
• 目標翻譯後修改: unmodified
• 基因資訊: human ... PMEPA1(56937)

描述

一般說明

Protein TMEPAI (UniProt: Q969W9; also known as Prostate transmembrane protein androgen induced 1, Solid tumor-associated 1 protein, Transmembrane prostate androgen-induced protein) is encoded by the PMEPA1 (also known as STAG1, TMEPAI) gene (Gene ID: 56937) in human. TMEPAI is a type Ib transmembrane protein containing two TMEPAI contains 2 WW-binding motifs and a SMAD interaction motif. The WW-binding motifs mediate its interaction with NEDD4 and the SMAD interaction motif is required for interaction with SMAD2 and SMAD3 and for the negative regulation of TGF-beta signaling. TMEPAI antagonizes TGF-beta signaling by interfering with TGF-beta type I receptor-induced R-SMAD phosphorylation. It competes with SMAD anchor for receptor activation for R-SMAD binding, thereby sequestering R-SMADs from TGF-beta receptor I kinase activation. By acting as a negative regulator of TGF-beta signaling, TMEPAI plays a role in cell proliferation, differentiation, apoptosis, motility, extracellular matrix production, and immunosuppression. Higher expression of TMEPAI is observed in prostate and ovaries and the expression can be up-regulated by androgen and TGF-beta (at protein level).TMEPAI is constitutively and highly expressed in many types of cancer and has been implicated in poor prognosis. TMEPAI is also reported to be involved in down-regulation of the androgen receptor (AR), enhancing ubiquitination and proteasome-mediated degradation of AR. (Ref.: Nguyen THV et al. (2014). Cancer Sci. 105: 334 341; Watanabe, Y et al. (2010). Mol. Cell 37(1): 123-134).

特異性

Clone 9F10 detects TMEPAI in COS-7 cells overexpressing TEMPAI. It targets an epitope of 39 amino acids from the C-terminal region in isoform 1. Also shows reactivity with isoform 2 that has 100% sequence homology in this epitope region.

免疫原

GST-tagged recombinant fragment corresponding to 39 amino acids from the C-terminal region of human TMEPAI.

應用

Immunoprecipitation Analysis: A representative lot detected TMEPAI in Immunoprecipitation applications (Vo Nguyen, T.T., et. al. (2014). Cancer Sci. 105(3):334-41).

Western Blotting Analysis: A representative lot detected TMEPAI in lysate from COS-7 cells overexpressing TEMPAI. (Courtesy of Yukihide Watanabe, Ph.D. and Mitsuyasu Kato, Ph.D., University of Tsukuba, Japan).

Western Blotting Analysis: 8 ug/mL from a representative lot detected TMEPAI in TGF-beta stimulated HaCaT cell lysates (Courtesy of Yukihide Watanabe, Ph.D. and Mitsuyasu Kato, Ph.D., University of Tsukuba, Japan).

Immunocytochemistry Analysis: A representative lot detected TMEPAI in Immunocytochemistry applications (Vo Nguyen, T.T., et. al. (2014). Cancer Sci. 105(3):334-41).

Western Blotting Analysis: A representative lot detected TMEPAI in Western Blotting applications (Vo Nguyen, T.T., et. al. (2014). Cancer Sci. 105(3):334-41).

Research Category
Cell Structure

This mouse monoclonal Anti-TMEPAI, Antibody, clone 9F10, Cat. No. MABS1905 is used in Immunocytochemistry, Immunoprecipitation, and Western blotting for the detection of TMEPAI.

品質

Evaluated by Western Blotting in COS-7 overexpressing TMEPAI lysate.

Western Blotting Analysis: 0.02 µg/mL of this antibody detected TMEPAI in 45 µg of lysate from COS-7 cells overexpressing TMEPAI.

標靶描述

~32 kDa observed; 31.61 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外觀

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1