MABS1683

Anti-UBE3A Antibody, clone 10H7.1

clone 10H7.1, from mouse

• 同義詞: Ubiquitin-protein ligase E3A, EC: 2.3.2.26, E6AP ubiquitin-protein ligase, HECT-type ubiquitin transferase E3A, Human papillomavirus E6-associated protein, Oncogenic protein-associated protein E6-AP, Renal carcinoma antigen NY-REN-54
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: 10H7.1, monoclonal
• 物種活性: human
• 包裝: antibody small pack of 25 μL
• 技術: immunohistochemistry: suitable (paraffin); western blot: suitable
• 同型: IgG2aκ
• NCBI登錄號: NP_000453
• UniProt登錄號: Q05086
• 運輸包裝: ambient
• 目標翻譯後修改: unmodified
• 基因資訊: human ... UBE3A(7337)

描述

一般說明

Ubiquitin-protein ligase E3A (UniProt: Q05086; also known as EC: 2.3.2.26, E6AP ubiquitin-protein ligase, HECT-type ubiquitin transferase E3A, Human papillomavirus E6-associated protein, Oncogenic protein-associated protein E6-AP, Renal carcinoma antigen NY-REN-54) is encoded by the UBE3A (also known as E6AP, EPVE6AP, HPVE6A) gene (Gene ID: 7337)in human. UBE3A is the E3 ubiquitin-protein ligase that accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified, including the RAD23A and RAD23B, MCM7, annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. UBE3A catalyzes the high-risk human papilloma virus (HPV) E6-mediated ubiquitination of p53/TP53, contributing to the neoplastic progression of cells infected by these viruses. Phosphorylation at Tyr659 by ABL1 impairs E3 ligase activity and protects p53/TP53 from degradation in (HPV)-infected cells. UBE3A may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins and it also promotes its own degradation in vivo. It is reported to plays an important role in the regulation of the circadian clock by ubiquitinating the core clock component ARNTL/BMAL1 and leading to its proteasomal degradation. Three isoforms of UBE3A have been reported that are generated via alternative splicing. Mutations in UBE3A gene cause Angelman syndrome that is characterized by severe motor and intellectual retardation, ataxia, hypotonia, and frequent jerky limb movements.

特異性

Clone 10H7.1 detects Ubiquitin-protein ligase E3A in human cells. it targets an epitope with in 101 amino acids from the internal region.

免疫原

GST/His-tagged recombinant fragment corresponding to 101 amino acids from the internal region of human Ubiquitin-protein ligase E3A.

應用

Anti-UBE3A, clone 10H7.1, Cat. No. MABS1683, is a mouse monoclonal antibody that detects Ubiquitin-protein ligase E3A and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected UBE3A in 10 µg of HeLa cell lysate.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected UBE3A in human cerebral cortex and human testis tissue.

品質

Evaluated by Western Blotting in PC-12 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected UBE3A in 10 µg of PC-12 cell lysate.

標靶描述

~100 kDa observed; 100.69 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外觀

Format: Purified

其他說明

Concentration: Please refer to lot specific datasheet.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1