MABS1286

Anti-EPO-R Antibody, clone BCO-3H2

clone BCO-3H2, from rat

• 同義詞: Erythropoietin receptor
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rat
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: BCO-3H2, monoclonal
• 物種活性: human
• 包裝: antibody small pack of 25 μg
• 技術: immunofluorescence: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• 同型: IgG2bκ
• NCBI登錄號: NP_000112
• UniProt登錄號: P19235
• 運輸包裝: ambient
• 目標翻譯後修改: unmodified
• 基因資訊: human ... EPOR(2057)

描述

一般說明

Erythropoietin receptor (UniProt: P19235; also known as EPO-R) is encoded by the EPOR gene (Gene ID: 2057) in human. EPO-R is a single-pass type 1 membrane protein that mediates erythropoietin-induced erythroblast proliferation and differentiation. It is synthesized with a signal peptide (aa 1-24) that is cleaved to produce the mature form. Upon EPO stimulation, EPO-R homodimerizes and gets phosphorylated on C-terminal tyrosine residues by JAK2. The phosphotyrosine motifs are also recruitment sites for several SH2-containing proteins and adapter proteins which mediate cell proliferation. The tyrosine-phosphorylated form interacts with several SH2 domain-containing proteins. The EPO-R is expressed in erythroid cells and erythroid progenitor cells. Three isoforms of EPO-R has been described that are produced by alternate splicing. Isoform EPOR-F is the most abundant form in EPO-dependent erythroleukemia cells and in late-stage erythroid progenitors. Isoform EPOR-S and isoform EPOR-T are the predominant forms in bone marrow. Isoform EPOR-T is the most abundant from in early-stage erythroid progenitor cells. Mutation in EPOR gene are linked to familial erythrocytosis that is characterized by elevated serum red blood cell mass, hemoglobin and hematocrit, and increased sensitivity of erythroid progenitors to erythropoietin.

特異性

Clone BCO-3H2 is a rat monoclonal antibody that detects erythopoietin receptor in human cells. It targets an epitope with in 17 amino acids from the C-terminal region.

免疫原

Epitope: cytoplasmic domain

KLH-conjugated linear peptide corresponding to 17 amino acids from the human erythropoietin receptor.

應用

Anti-EPO-R, clone BCO-3H2, Cat. No. MABS1286, is a rat monoclonal antibody that detects erythropoietin receptor and has been tested for use in Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blotting.

Immunohistochemistry Analysis: A representative lot detected EPO-R in Immunohistochemistry applications (Maxwell, P., et. al. (2015). Br J Haematol. 168(3):429-42).

Immunoprecipitation Analysis: A representative lot detected EPO-R in Immunoprecipitation applications (Maxwell, P., et. al. (2015). Br J Haematol. 168(3):429-42).

Immunofluorescence Analysis: A representative lot detected EPO-R in Immunofluorescence applications (Maxwell, P., et. al. (2015). Br J Haematol. 168(3):429-42).

Western Blotting Analysis: A representative lot detected EPO-R in Western Blotting applications (Maxwell, P., et. al. (2015). Br J Haematol. 168(3):429-42).

Research Category
Signaling

品質

Evaluated by Western Blotting in UT-7 cell lysates.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected EPO-R in 10 µg of UT-7 cell lysates.

標靶描述

~58 kDa observed; 55.07 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外觀

Protein G purified

Format: Purified

Purified rat monoclonal antibody IgG2b in PBS without azide.

儲存和穩定性

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable