MABS1215

Anti-Calpain I/II, large subunit Antibody, clone 1D10A7

ascites fluid, clone 1D10A7, from mouse

• 同義詞: Calpain-1 catalytic subunit, Calcium-activated neutral proteinase 1, CANP 1, Calpain mu-type, Calpain-1 large subunit, Cell proliferation-inducing gene 30 protein, Micromolar-calpain, muCANP, Calpain I/II large subunit
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: ascites fluid
• 抗體產品種類: primary antibodies
• 無性繁殖: 1D10A7, monoclonal
• 物種活性: human, rat, rabbit, monkey, chicken
• 技術: immunocytochemistry: suitable; western blot: suitable
• 同型: IgG2aκ
• NCBI登錄號: NP_001185797
• UniProt登錄號: P07384
• 運輸包裝: dry ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... CAPN1(823)

描述

一般說明

Calpains I & II are intracellular cysteine proteinases distributed in various tissues and cells. Calpain I & II are also known as mu- and m-calpains, respectively, based on their calcium requirement, with calpain I being active at micromolar Ca+2 concentrations and calpain II at millimolar Ca+2 concentrations. Both calpains are composed of a large catalytic (80 kDa) and a small regulatory (30 kDa) subunit, and each subunit contains a calmodulinlike domain at the C-terminus. Upon Ca2+ binding to the calmodulin-like domains, calpains become activated for catalyzing both substrate proteolytic degradation as well as autolysis. Fourteen calpain large subunits have been identified in human (calpain 1-3 & 5-15 encoded by CAPN1-3 & 5-15 gene) and two small subunits encoded by the CAPNS1 & CAPSN2 genes. The calpain small subunit 1 encoded by CAPNS1 is also known as calpain 4. To date, calpain I & II remain the best characterized members of the calpain family. Calpain activity was initially characterized as “calcium-activated neutral protease” (CANP) detected in brain, lens of the eye, and other tissues. The name “calpain” was later adopted as a hybrid name from two well-known proteins at the time, calmodulin and papain.

特異性

Clone 1D10A7 detects the large catalytic (80K), but not the small regulatory (30K), subunit of mu-calpain (calpain I) and m-calpain (calpain II), with ~7-fold higher affinity toward m-calpain than mu-calpain.

免疫原

Purified m-calpain from rabbit skeletal muscle.

應用

Detect CAPN1 using this mouse monoclonal Anti-Calpain I/II, large subunit Antibody, clone 1D10A7, Cat. No. MABS1215, validated for use in Western Blotting and Immunocytochemistry.

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

Western Blotting Analysis: A 1:20,000 dilution from a representative lot detected recombinant human calpain-2 and calpain-1 C115S mutant protein.
Western Blotting Analysis: A 1:20,000 dilution from a representative lot detected Calpain, large subunit in rat spleen tissue lysate.
Immunocytochemistry Analysis: A representative lot detected Calpain, large subunit in mouse embryonic fibroblast (MEF) cells (Yamada, M., et al. (2009). Nat Med. 15(10):1202-1207).
Western Blotting Analysis: A representative lot detected Calpain, large subunit in cytosolic preparation from rat cortical tissue (Blomgren, K., et al. (1995). Brain Res. 684(2):143-149).
Western Blotting Analysis: A representative lot detected purified mu- and m-calpain large subunits from rabbit, monkey, human, and rat tissues (Kawashima, S., et al. (1998). Biol Chem. 379(2):201-204).
Western Blotting Analysis: A representative lot detected Calpain, large subunit in normal & cataractous rat lens homogenates (Inomata, M., et al. (2009). Nat Med. 15(10):1202-1207).
Western Blotting Analysis: A representative lot detected Calpain, large subunit in MEF cells (Yamada, M., et al. (2009). Nat Med. 15(10):1202-1207).

品質

Evaluated by Western Blotting in rat lung tissue lysate.

Western Blotting Analysis: A 1:20,000 dilution of this antibody detected Calpain, large subunit in rat lung tissue lysate.

標靶描述

~80 kDa observed. Uncharacterized band(s) may appear in some lysates.

外觀

Unpurified

Mouse monoclonal IgG2aκ ascites with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: nwg
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable