MABS1171

Anti-MPST Antibody, clone 1H10.1

clone 1H10.1, from mouse

• 同義詞: 3-mercaptopyruvate sulfurtransferase, MpST, MST, tRNA thiouridine modification protein, TUM1
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: 1H10.1, monoclonal
• 物種活性: human
• 技術: immunohistochemistry: suitable (paraffin); western blot: suitable
• 同型: IgG2aκ
• NCBI登錄號: NP_001013454
• UniProt登錄號: P25325
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... MPST(4357)

描述

一般說明

3-mercaptopyruvate sulfurtransferase (EC 2.8.1.2; UniProt P25325; also known as MpST, MST, tRNA thiouridine modification protein, TUM1) is encoded by the MPST (also known as TST2) gene (Gene ID 4357) in human. 3-mercaptopyruvate sulfurtransferase (MpST) catalyzes the transfer of a sulfur ion to cyanide or other thiol compounds and plays a central role in both cysteine degradation and cyanide detoxification. MpST is also known as tRNA thiouridine modification protein (TUM1) and is implicated in other cellular processes in addition to cyanide detoxification, ranging from thiolation of cytosolic tRNAs to the generation of H2S as signaling molecule both in mitochondria and the cytosol. Chronic cyanide intoxications caused by cyanogenic substances present in food products can lead to neurological disorders. MpST and rhodanese [EC 2.8.1.1] are two enzymes that catalyze cyanide detoxification, with MpST also displaying weak rhodanese activity. Cyanide detoxification by rhodanese requires the presence of compounds bearing labile sulfane sulfur, while mercaptopyruvate is needed for MpST catalyzed reaction. Under oxidative stress conditions, the catalytic cysteine site is converted to a sulfenate, resulting in an inactivation of MpST enzymatic activity. Reduced thioredoxin reactivates the enzyme by cleaving an intersubunit disulfide bond to turn the redox switch on. Aberrant MpST activity is found in a few cases of mercaptolactate-cysteine disulfiduria (MCDU) characterized by the appearance of large quantaties of the sulfur-containing amino acid, beta-mercaptolactate-cysteine disulfide, in the urine.

特異性

Clone 1H10.1 targets an epitope in the second Rhodanese domain present in both spliced isoforms of human MPST reported by UniProt (P25325).

免疫原

Epitope: Second Rhodanese domain.

GST-tagged recombinant fragment from the second Rhodanese domain of human MPST.

應用

Research Category
Signaling

Research Sub Category
Toxicology & Drug Resistance

This Anti-MPST Antibody, clone 1H10.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of MPST.

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected MPST in 10 µg of human liver tissue lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected MPST in human heart and kidney tissue sections.

品質

Evaluated by Western Blotting in SHSY-5Y cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected MPST in 10 µg of SHSY-5Y cell lysate.

標靶描述

~33 kDa observed. 33.05 kDa (isoform 1) and 35.12 kDa (isoform 2) calculated (Met1 removed). Uncharacterized band(s) may appear in some lysates.

外觀

Protein G purified.

Format: Purified

Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1