MABS1163

Anti-CSF-1R/CD115 Antibody clone FER216

clone FER216, from mouse

• 同義詞: Macrophage colony-stimulating factor 1 receptor, CD115, CSF-1 receptor, CSF-1-R, CSF-1R, M-CSF-R, Proto-oncogene c-Fms
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: FER216, monoclonal
• 物種活性: human
• 技術: western blot: suitable
• 同型: IgG1κ
• NCBI登錄號: NP_005202
• UniProt登錄號: P07333
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... CSF1R(1436)

描述

一般說明

Macrophage colony-stimulating factor 1 receptor (EC 2.7.10.1; UniProt P07333; also known as CD115, CSF-1 receptor, CSF-1-R, CSF-1R, M-CSF-R, Proto-oncogene c-Fms) is encoded by the CSF1R (also known as CSFR, FMS, HDLS) gene (Gene ID 1436) in human. CSF1R is a CSF1/PDGF family receptor tyrosine kinase that mediates macrophage colony stimulating factor 1 (M-CSF1) and IL-34 signaling by acting as the receptor for these two cytokines. CSF1R plays a key role in the differentiation, recruitment and activation of tissue macrophages. Tumor-associated macrophages (TAMs) have been associated with survival in classic Hodgkin lymphoma (cHL) and other lymphoma types. Hyperphosphorylated CSF1R is found in cHL tissue and highlights the importance of CSF1/CSF1R signaling in the recruitment of TAMs. CSF1R is initially produced with a signal peptide sequence (a.a. 1-19), the removal of which yields the mature type I single transmembrane protein with an extracellular region (a.a. 20-517), a transmembrane segment (a.a. a.a. 518-538), and a cytoplasmic region (a.a. 539-972) that contains the kinase domain (a.a. 582-910).

特異性

Clone FER216 reacts with the extracellular domain of human CSFR1, but not the extracellular domain of human FGFR1. Clone FER216 detected the ~100 kDa human CSFR1 in nomral tonsil and Hodgkin lymphoma (cHL) tissue extracts, as well as an additional ~150 kDa phosphorylated CSFR1 band in cHL tissue extracts (Martín-Moreno, A.M., et al. (2015). PLoS One. 2015 Jun 12;10(6):e0125203).

免疫原

Epitope: Extracellular domain.

Human IgG1 Fc-tagged recombinant human CSF-1R/CD115 extracellular domain.

應用

Anti-CSF-1R/CD115 Antibody clone FER216 is an antibody against CSF-1R for use in Western Blotting.

Research Category
Signaling

Research Sub Category
Cytokines & Cytokine Receptors

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected CSF-1R/CD115 in 10 µg of human tonsil tissue lysate.

品質

Evaluated by Western Blotting in human colon tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected CSF-1R/CD115 in 10 µg of human colon tissue lysate.

標靶描述

~107 kDa observed. Uncharacterized band(s) may appear in some lysates.

外觀

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable