MABN726

Anti-EphB2 Antibody, clone 2D12C6

ascites fluid, clone 2D12C6, from mouse

• 同義詞: Ephrin type-B receptor 2, EPHB2 protein
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• 無性繁殖: 2D12C6, monoclonal
• application: ICC; IHC; WB
• 物種活性: rat, human
• 技術: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: ascites fluid
• 抗體產品種類: primary antibodies
• 無性繁殖: 2D12C6, monoclonal
• 物種活性: rat, human
• 技術: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• 同型: IgG2b
• UniProt登錄號: Q6NVW1
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... EPHB2(2048) , EphB2(2048) ; rat ... EphB2(313633)

描述

一般說明

Ephrin type-B receptor 2 (EphB2) is a receptor tyrosine kinase for ephrin ligands that mediate numerous developmental processes, particularly in the nervous system. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. EphB2 is critical for embryonic/postnatal development for lateral suprapyramidal blade formation which is in a specific region of the dentate gyrus. EphB2 is expressed in the two main components of the thymus, developing thymocytes, TEC, colorectal cancer, and in the earliest SGZ progenitor cells as they migrate during development and form the dentate gyrus.

免疫原

Purified recombinant fragment of EphB2 expressed in E. coli.

應用

Anti-EphB2 Antibody, clone 2D12C6 is a highly specific mouse monoclonal antibody, that targets EphB2 & has been tested in western blotting, IHC & ICC.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected EphB2 in HeLa and HepG2 cells.

Immunohistochemistry Analysis: 1:200 to 1:1,000 dilution from a representative lot detected EphB2 in rat cerebellum, pons/midbrain, and hippocampus tissues. Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

品質

Evaluated by Western Blot in IMR-32 cell lysate.

Western Blot Analysis: A 1:500 dilution of this antibody detected EphB2 in 10 µg of IMR-32 cell lysate.

標靶描述

~53 kDa observed

外觀

Unpurified

Mouse monoclonal IgG2b ascitic fluid containing up to 0.1% sodium azide.

儲存和穩定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析報告

Control
IMR-32 cell lysate

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: nwg
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable