MABN491

Anti-Brevican Antibody, clone N294A/6

clone N294A/6, from mouse

• 同義詞: Brevican core protein, Brain-enriched hyaluronan-binding protein, BEHAB, Brevican
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: N294A/6, monoclonal
• 物種活性: rat, human
• 技術: immunohistochemistry: suitable; western blot: suitable
• 同型: IgG2bκ
• NCBI登錄號: NP_001028837
• UniProt登錄號: P55068
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... BCAN(63827)

描述

一般說明

Brevican core protein (BCAN), also known as Brain-enriched hyaluronan-binding protein (BEHAB) or Chondroitin sulfate proteoglycan 7 (CSPG7), may play a role in the terminally differentiating and the adult nervous system during postnatal development. It may stabilize interactions between hyaluronan (HA) and brain proteoglycans. Two isoforms exist, with isoform 1 (~99 kDa) being highly expressed from birth through 8 years of age and down-regulated by 20 years of age to low levels that are maintained in the normal adult cortex; and isoform 2 (~72 kDa) being expressed at uniformly low levels throughout development.

免疫原

Recombinant protein corresponding to rat Brevican.

應用

Anti-Brevican, clone N294A/6 is an antibody targeting the Brevican protein, validated for use in WB & IHC.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

Western Blot Analysis: 2 µg/mL from a representative lot detected Brevican in 10 µg of human brain tissue lysate.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Brevican in rat midbrain, rat thalamus, and rat cerebellum tissues.

Western Blot Analysis: A representative lot detected Brevican in rat brain tissue lysate and in WT, but not in Brevican knockout mouse brain tissue lysate.

品質

Evaluated by Western Blot in rat brain tissue lysate.

Western Blot Analysis: 2 µg/mL of this antibody detected Brevican in 10 µg of rat brain tissue lysate.

標靶描述

~140 kDa observed. The calculated molecular weight of this protein is 96 kDa A second isoform is known to exist at 69 kDa This protein may be observed at a higher molecular weight due to glycosylation. A proteolyticaly cleaved fragment of Brevican may be observed at ~50 kDa in some tissue lysates.

外觀

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

分析報告

Control
Rat brain tissue lysate

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable