MABN2463

Anti-KCNT1 Antibody

• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: N3/26, monoclonal
• 分子量: calculated mol wt 139.62 kDa; observed mol wt ~140 kDa
• 物種活性: rat
• 包裝: antibody small pack of 100 μL
• 技術: immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• 同型: IgG1κ
• UniProt登錄號: Q9Z258
• 運輸包裝: dry ice
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: rat ... Kcnt1(60444)

描述

一般說明

Potassium channel subfamily T member 1 (UniProt: Q9Z258; also known as Sequence like a calcium-activated potassium channel subunit, Slack, KCNT1) is encoded by the Kcnt1 (also known as Slack) gene (Gene ID: 60444) in rat. KCNT1 is a multi-pass membrane protein that serves as an outwardly rectifying potassium channel subunit that may co-assemble with other Slo-type channel subunits. It represents the largest known potassium channel subunit. It is activated by high intracellular sodium or chloride levels and is activated upon stimulation of G-protein coupled receptors, such as CHRM1 and GRIA1. KCNT1 has four cytoplasmic domains, six transmembrane domains, and four extracellular domains. It is widely expressed in the nervous system and its activity contributes to the slow hyperpolarization that follows repetitive firing. KCNT1 is phosphorylated by protein kinase C and phosphorylation of the C-terminal domain is shown to increase its channel activity by 2 to 3-folds. Mutations in Kcnt1 gene have been linked to malignant migrating partial seizures of infancy (MMPSI), a rare epileptic encephalopathy of infancy. Two isoforms of KCNT1 have been described that are produced by alternative splicing. (Ref.: Barcia, G., et al. (2012). Nat. Gen. 44(11); 1255-1259).

特異性

Clone N3/26 is a mouse monoclonal antibody that detects Potassium channel subfamily member 1 (KCNT1). It targets an epitope within 70 amino acids from the C-terminal region.

免疫原

Fusion protein corresponding to 70 amino acids from the C-terminal, cytoplasmic domain of rat Potassium channel subfamily member 1 (KCNT1).

應用

Quality Control Testing

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected KCNT1 in rat brain tissue lysate.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected KCNT1 in rat brain tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-KCNT1, clone N3/26, Cat. No. MABN2463, is a mouse monoclonal antibody that detects Potassium channel subfamily T member 1 (KCNT1) and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

外觀

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C . Avoid repeated freeze-thaws.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable