MABN1844

Anti-Aldolase C Antibody, C-Term, clone 1A1

clone 1A1, from mouse

• 同義詞: Fructose-bisphosphate aldolase C, Brain-type aldolase, Aldolase C, C-Term
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: 1A1, monoclonal
• 物種活性: human, rat
• 技術: immunocytochemistry: suitable; western blot: suitable
• 同型: IgG1κ
• NCBI登錄號: NP_005156
• UniProt登錄號: P09972
• 運輸包裝: dry ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... ALDOC(230)

描述

一般說明

Fructose-bisphosphate aldolase C (EC 4.1.2.13; UniProt P09972; also known as Aldolase 3, Brain-type aldolasefructoaldolase C, Fructose-1,6-biphosphate triosephosphate lyase) is encoded by the ALDOC (also known as ALDC) gene (Gene ID 230) in human. Aldolases are glycolytic enzymes that catalyze the reversible aldol cleavage of fructose 1,6-bisphosphate and fructose-1-phosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. Three aldolase genes (ALDOA, ALDOB, ALDOC) are found in mammals that encode aldolase A, B, and C. Aldolase A is generally considered to be a muscle enzyme, aldolase B a liver enzyme, while aldolase C shows tissue-specific expression in adult brain with low activity in fetal tissues. Aldolase C expression is found in gray matter astrocytes and cells of the pia mater. Aldolase A and C mRNA expressions are mutually exclusive in murine central nervous system by in situ hybridization analysis, with aldolase A mRNA being detected in neurons and aldolase C in astrocytes, Purkinje cells, and some cells of the pia mater. Aldolase C can therefore be a useful marker of astrocytes, although Purkinje cells of the cerebellum also contain high levels of aldolase C.

免疫原

Epitope: Near C-terminus; 23 amino acids of aldolase C protein

Recombinant protein corresponding to human Aldolase C near the C-terminus.

應用

This Anti-Aldolase C Antibody, C-Term, clone 1A1 is validated for use in Western Blotting, Immunocytochemistry for the detection of Aldolase C.

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Aldolase C in 10 µg of human brain tissue lysate.
Immunocytochemistry: A representative lot selectively stained Aldolase C-positive astrocytes in mixed neuron/glial cultures.

品質

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Aldolase C in 10 µg of rat brain tissue lysate.

標靶描述

~39 kDa observed

外觀

Format: Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing PBS with 0.05% sodium azide with 50% glycerol.

其他說明

Concentration: Please refer to lot specific datasheet.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable