MABN1833
Anti-nAChR α4 Subunit Antibody, clone 369
clone 369, from mouse
同義詞:
Neuronal acetylcholine receptor subunit alpha-4, alpha4 nAChR subunit, Cholinergic receptor, nicotinic, alpha polypeptide 4, nAChR alpha4 subunit, Neuronal nicotinic acetylcholine receptor alpha-4 subunit
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About This Item
分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41
推薦產品
生物源
mouse
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
369, monoclonal
物種活性
monkey, human, rabbit
技術
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
同型
IgG1κ
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... CHRNA4(1137)
一般說明
Neuronal acetylcholine receptor subunit alpha-4 (UniProt P43681; also known as alpha4 nAChR subunit, cholinergic receptor, nicotinic, alpha polypeptide 4, nAChR alpha4 subunit, Neuronal nicotinic acetylcholine receptor alpha-4 subunit) is encoded by the CHRNA4 (also known as NACRA4, NACHRA4) gene (Gene ID 1137) in human. The nicotinic acetylcholine (ACh) receptors (nAChRs) are activated by acetylcholine (ACh) and nicotinic agonists, such as cytisine, 1,1-dimethyl-4-phenylpiperazinium and carbachol. The nAChRs are ligand-gated pentameric cation channels. Currently, 12 neuronal and 5 muscle nAChR subunits have been identified. The nAChRs can exist as homopentamers of five identical alpha subunits (α7, α8, or α9), alpha heteropentamers composed of more than one type of alpha subunits (α7-α10), or as alpha/beta heteropentamers consisting of various combinations of alpha (α2-α6) and beta (β2-β4) subunits. The heteromeric alpha/beta types display high ligand affinity, but are insensitive to α-bungarotoxin (αBgt), while the alpha-only homomeric and heteromeric pentamers show lower agonist affinity, but high αBgt sensitivity. The α4β2 (two α4 and three β2 subunits) and α7 (five α7 subunits) nAChRs are the predominant nAChRs in the central nervous system (CNS) among mammalian species. The alpha-4 subunit is initially produced with a signal peptide (a.a. 1-28), the removal of which yields the mature 627-amino acid membrane protein with a large extracellular domain (a.a. 29-242), four transmembrane segments (a.a. 243-267, 275-293, 309-330, 601-619), and a large cytoplasmic loop (a.a. 331-600) between the third and fourth transmembrane domains.
免疫原
Recombinant human nAChR α4 subunit.
應用
Anti-nAChR α4 Subunit Antibody, clone 369 is an antibody against nAChR α4 Subunit for use in Immunohistochemistry (Paraffin), Immunofluorescence.
Immunohistochemistry Analysis: An 1:50 dilution from a representative lot detected nAChR α4 Subunit in human small intestine tissue.
Immunofluorescence Analysis: A representative lot detected the localization of nAChR α4 subunit immunoreactivity in monkey frozen retina sections by fluorescence immunohistochemistry (Liu, J., et al. (2009). Invest. Ophthalmol. Vis .Sci. 50(3):1408-1415).
Immunofluorescence Analysis: A representative lot detected the localization of nAChR α4 subunit immunoreactivity in various areas of the rabbit brainstem by fluorescence immunohistochemical staining of free-floating sections (Centeno, M.L., et al. (2004). Brain Res. 1012(1-2):1-12).
Immunofluorescence Analysis: A represenative lot detected an increase of nAChR α4-positive neurons in the A1, A2 and A6 brainstem noradrenergic areas of female rabbits 1 hr after mating by fluorescence immunohistochemical staining of free-floating sections. The number of nAChR α4-positive neurons subsided to basal level 2 hrs after mating (Centeno, M.L., et al. (2004). Brain Res. 1012(1-2):1-12).
Immunofluorescence Analysis: A representative lot detected the localization of nAChR α4 subunit immunoreactivity in monkey frozen retina sections by fluorescence immunohistochemistry (Liu, J., et al. (2009). Invest. Ophthalmol. Vis .Sci. 50(3):1408-1415).
Immunofluorescence Analysis: A representative lot detected the localization of nAChR α4 subunit immunoreactivity in various areas of the rabbit brainstem by fluorescence immunohistochemical staining of free-floating sections (Centeno, M.L., et al. (2004). Brain Res. 1012(1-2):1-12).
Immunofluorescence Analysis: A represenative lot detected an increase of nAChR α4-positive neurons in the A1, A2 and A6 brainstem noradrenergic areas of female rabbits 1 hr after mating by fluorescence immunohistochemical staining of free-floating sections. The number of nAChR α4-positive neurons subsided to basal level 2 hrs after mating (Centeno, M.L., et al. (2004). Brain Res. 1012(1-2):1-12).
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neurotransmitters & Receptors
品質
Evaluated by Immunohistochemistry in human cerebellum tissue.
Immunohistochemistry Analysis: An 1:50 dilution of this antibody detected nAChR α4 Subunit in human cerebellum tissue.
Immunohistochemistry Analysis: An 1:50 dilution of this antibody detected nAChR α4 Subunit in human cerebellum tissue.
標靶描述
67.16 kDa calculated.
外觀
Protein G Purified
Format: Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
儲存和穩定性
Stable for 1 year at 2-8°C from date of receipt.
其他說明
Concentration: Please refer to lot specific datasheet.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
分析證明 (COA)
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