MABN1596

Anti-Pan Shc Antibody, clone 3A12.1

clone 3A12.1, from mouse

• 同義詞: p46Shc, p52Shc, p66Shc, SHC-transforming protein 1, SH2 domain protein C1, SHC-transforming protein 3, SHC-transforming protein A, Src homology 2 domain-containing-transforming protein C1
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: 3A12.1, monoclonal
• 物種活性: human
• 技術: immunohistochemistry: suitable; western blot: suitable
• 同型: IgG2aκ
• NCBI登錄號: NP_001123512
• UniProt登錄號: P29353
• 目標翻譯後修改: unmodified
• 基因資訊: human ... SHC1(6464)

描述

一般說明

SHC-transforming protein 1 (UniProt P29353; also known as SH2 domain protein C1, SHC-transforming protein 3, SHC-transforming protein A, Src homology 2 domain-containing-transforming protein C1) is encoded by the SHC1 (also known as SHC, SHCA) gene (Gene ID 6464) in human. Originally identified as an SH2-containing proto-oncogene involved in growth factor signaling, Shc proteins function as adaptor molecules recruited by numerous receptors (e.g. integrins, GPCRs, and receptors for growth factors, antigens, cytokines, and hormones) for signling. In addition to the ubiquitously expressed Shc (a.k.a. ShcA by SHC1/SHCA gene), there exist also ShcB (by SHC2/SHCB gene) and ShcC (by SHC3/SHCC gene), which are predominantly expressed in neuronal cells. Alternative splicings result in six ShcA isoforms, including p66Shc, p52Shc, and p46Shc. All ShcA isoforms possess two distinct domains, PTB (a.a. 156-339) and SH2 (a.a. 488-579), that bind phosphotyrosine-containing sequences, as well as a central region (CH1; a.a. 340-487) that contains critical tyrosine phosphorylation sites. The phosphotyrosine-binding (PTB) domain is also referred to as phosphotyrosine-interaction domain or PID.

特異性

Clone 3A12.1 was raised against a C-terminal region sequence present in all six human SHC1 spliced isoforms reported by UniProt (P29353).

免疫原

GST-tagged recombinant human Shc C-terminal region fragment.

應用

Anti-Pan Shc Antibody, clone 3A12.1 is an antibody against Pan Shc for use in Western Blotting, Immunohistochemistry.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Shc proteins in human prostate cancer, as well as in normal breast and tonsil tissue sections.

品質

Evaluated by Western Blotting in SH-SY5Y cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Shc proteins in 10 µg of SH-SY5Y cell lysate.

標靶描述

~47-63 kDa observed. 62.82 kDa (isoform p66Shc), 51.61 kDa (isoform p52Shc), 46.67 kDa (isoform p46Shc), 40.42 kDa (isoform 5), 62.89 kDa (isoform 6), 51.68 kDa (isoform 7) calculated.

外觀

Format: Purified

其他說明

Concentration: Please refer to lot specific datasheet.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable