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Merck
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Key Documents

MABF557

Sigma-Aldrich

Anti-CD28 Antibody (human), APC, clone CD28.2

clone CD28.2, from mouse

同義詞:

T-cell-specific surface glycoprotein CD28, TP44, CD antigen CD28

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

mouse

品質等級

共軛

Allophycocyanin conjugate

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

CD28.2, monoclonal

物種活性

human

包裝

pkg of 5 μL (0.25 μg/test)

技術

flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

同型

IgG1κ

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... CD28(940)

一般說明

The CD28.2 antibody reacts with human CD28, a 44 kDa type I surface glycoprotein which acts as a co-stimulatory receptor in support of the T cell receptor (TCR). CD28 exists as a homodimer with specificity for two known ligands, known as B7-1 (CD80) and B7-2 (CD86), which are expressed on activated B cells and antigen-presenting cells. These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction / co-stimulatory signaling between the B7 ligands and CD28 provides crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response. Other members of the CD28 family of receptors include CTLA-4 (CD152), PD-1 (CD279), ICOS and BTLA.The CD28.2 antibody may be used as a phenotypic marker for human CD28, expressed on all CD4+ T cells and CD8+ T cells, and is widely used as a reagent for activation of the CD28 receptor in vitro and in vivo. This antibody is also reported to be cross-reactive with several non-human species, including Baboon, Chimpanzee, Cynomolgus, and Rhesus.

免疫原

Immunogen information for this antibody is proprietary information.

應用

Research Category
Inflammation & Immunology
This mouse monoclonal antibody Anti-CD28 (human), APC & clone CD28.2 validated for use in IP;IHC;Flow Cytometry for the detection of CD28.

品質

The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.25 µg of this antibody detected CD28 in one million human peripheral blood lymphocytes.

外觀

Size Exclusion Chromatography
Purified mouse monoclonal antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.

儲存和穩定性

It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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