MABF3149

Anti-Rabies Virus Phosphoprotein Antibody, clone M957

• 同義詞: Protein P;Protein M1
• 分類程式碼代碼: 12352203
• NACRES: NA.43

屬性

• 生物源: mouse
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: M957, monoclonal
• 分子量: calculated mol wt 33.21 kDa; observed mol wt ~44 kDa
• 純化經由: using protein G
• 物種活性: rabies virus
• 包裝: antibody small pack of 100 μg
• 技術: ELISA: suitable; immunohistochemistry (frozen sections): suitable; inhibition assay: suitable; western blot: suitable
• 同型: IgG1κ
• 表位序列: N-terminal half
• UniProt登錄號: P69480
• 運輸包裝: dry ice
• 目標翻譯後修改: unmodified

描述

一般說明

Phosphoprotein (UniProt: P69480; also known as Protein P, Protein M1) is encoded by the P gene in Rabies virus. Rabies virus belongs to a family of RNA viruses called Rhabdoviridae, genus Lyssavirus. It is most commonly transmitted to humans through the saliva of an infected animal. This genus of virus shares many of the biological and physicochemical features that are associated with other rhabdoviruses. They have similar mechanisms to enter susceptible cells, express and replicate their genome RNA and release mature virus particles from the plasma membrane of infected cells. Rabies virus prototype is organized into five coding regions, N, P, M, G, and L. Viral transcription and replication are catalyzed by the viral polymerase complex, composed of the L and P proteins. Rabies virus is reported to bind to nicotinic acetylcholine receptors at the neuromuscular junction and travels toward the spinal cord within axons of peripheral nerves by retrograde fast axonal transport. The virus disseminates within axons in the CNS along neuroanatomical pathways. It replicates in neurons and causes neuronal dysfunction. The phosphoprotein of Rabies virus is a non-catalytic polymerase cofactor and regulatory protein that plays a role in viral transcription and replication. It is a multifunctional protein that is indispensable not only for viral replication but also for evasion of host innate immunity. It establishes a trimeric form when phosphorylated. It is reported to be phosphorylated at serine 162, 210, and 271 by protein kinase C. The trimmer is stabilized by binding to the L protein. It also binds to the soluble protein N, preventing it from encapsidating non-genomic RNA. It is reported to interact with host DYNLL1 and DYNLL2 via amino acids 138-172 and this interaction may play a role in intracellular microtubule-dependent virus transport of incoming virus. It is also reported to inhibits host IFN- and IFN-β signaling by binding and retaining phosp+D11horylated STAT1 in the cytoplasm or by inhibiting the DNA binding of STAT1 in the nucleus. Five isoforms of Rabies virus phosphoprotein have been described that are produced by alternative initiation. Clone M957 binds to the antigenic site consisting of amino acids 37-53 in the native P protein. (Ref.: Okada, K., et al. (2016). J. Virol. 90(18); 8226-8237; Nadin-Davis, SA., et al. (2000). J. Clin. Microbiol. 38(4); 1397-1403).

特異性

Clone M957 is a mouse monoclonal antibody that detects phosphoprotein of Rabies virus. It targets an epitope within 30 amino acids from the N-terminal half.

免疫原

GST-tagged recombinant phosphoprotein from Rabies virus, strain Arctic Fox type I, expressed in E.coli.

應用

Quality Control Testing

Evaluated by Western Blotting with recombinant Rabies Virus Phosphoprotein.

Western Blotting Analysis: 1:1 million (1.0 ng/mL) dilution of this antibody detected recombinant Rabies Virus Phosphoprotein.

Tested applications

Immunohistochemistry Applications: A representative lot detected Rabies Virus Phosphoprotein in Immunohistochemistry applications (Levinthal, D.J., et al. (2020). Proc Natl Sci USA. 117(23):13078-13083).

ELISA Analysis: A representative lot detected Rabies Virus Phosphoprotein in ELISA applications (Nadin-Davis, S.A., et al. (2000). J Clin Microbiol. 38(4):1397-1403).

Western Blotting Analysis: A representative lot detected Rabies Virus Phosphoprotein in Western Blotting applications (Nadin-Davis, S.A., et al. (2000). J Clin Microbiol. 38(4):1397-1403).

Inhibition Assay: A representative lot of this antibody inhibited binding of Rabies virus phosphoprotein in a competitive binding assay. (Nadin-Davis, S.A., et al. (2000). J Clin Microbiol. 38(4):1397-1403).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Rabies Virus Phosphoprotein, clone M957, Cat. No. MABF3149, is a mouse monoclonal antibody that detects Rabies Virus Phosphoprotein and is tested for use in ELISA, Immunohistochemistry, Inhibition Assay, and Western Blotting.

外觀

Purified mouse monoclonal antibody IgG1 in PBS without azide.

儲存和穩定性

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable