MABF3090

Anti-SARS-CoV-2 Spike N501Y Antibody, clone 6D4-E2

• 同義詞: S glycoprotein Alpha N501Y, Spike glycoprotein Alpha N501Y
• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 200
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 6D4-E2, monoclonal
• 分子量: calculated mol wt 141.18 kDa; observed mol wt ~200 kDa
• 純化經由: using protein G
• 物種活性: virus
• 包裝: antibody small pack of 100 μL
• 技術: immunoprecipitation (IP): suitable; western blot: suitable
• 同型: IgG1κ
• 表位序列: N-terminal half
• UniProt登錄號: P0DTC2
• 儲存溫度: 2-8°C
• 基因資訊: vaccinia virus ... S(43740568)

描述

特異性

Clone 6D4-E2 is a mouse monoclonal antibody that detects the alpha variant of SARS-CoV-2 with N501Y mutation in Spike protein.

免疫原

A recombinant fusion protein with a scaffold containing a short spike peptide GFQPTYGVGYQ.

應用

Quality Control Testing

Evaluated by Western Blotting in lysate from HEK293T cells transiently transfected with SARS-CoV-2 spike B.1.351, beta.

Western Blotting Analysis: A 1:500 dilution of this antibody detected SARS-CoV-2 spike protein with N501Y mutation in lysate from HEK293T cells transiently transfected with SARS-CoV-2 spike B.1.351, Beta, but not in wild-type HEK293T cells.

Tested Applications

Western Blotting Analysis: A representative lot detected SARS-CoV-2 spike N501Y in HEK293T transiently transfected with various SARS-CoV-2 variants (Data courtesy of Stefan Schüchner and Egon Ogris (Max Perutz Labs, Medical University of Vienna, Austria).

Immunoprecipitation Analysis: A representative lot detected SARS-CoV-2 spike N501Y in Whole cell lysates of HEK293T cells (untransfected or transiently transfected with SARS-CoV-2 spike WT or B.1.351 variant) (Data courtesy of Stefan Schüchner and Egon Ogris (Max Perutz Labs, Medical University of Vienna, Austria).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

標靶描述

Spike glycoprotein (UniProt: P0DTC2; also known as S glycoprotein, E2, Peplomer protein) is encoded by the S (also known as 2) gene (Gene ID: 43740568) in Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The SARS-CoV-2 is a positive-strand RNA virus that causes severe respiratory syndrome in human. The mature SARS-CoV-2 contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S). E and M proteins help in viral assembly and N protein is needed for RNA synthesis. The S protein is a single-pass type I, homotrimeric, membrane glycoprotein that is responsible for virus binding and entry into host cell. It is synthesized with a signal peptide (aa 1-12), which is subsequently cleaved off to generate the mature protein that contains an extracellular domain (aa 13-1213), a transmembrane domain (aa 1214-1234), and a cytoplasmic domain 1235-1273). The S protein is further processed into S1 (aa 13-685) and S2 (aa 686-1273) subunits by host cell furin. The presence of a furin polybasic cleavage site in S protein from SARS-CoV-2 sets it apart from S protein in SARS-CoV that possesses a monobasic S1/S2 cleavage site. The S1 subunit has the receptor binding domain (RBD; aa 319-541) that mediates entry of SARS-CoV-2 into sensitive cells through the peptidase domain of host Angiotensin-converting enzyme 2 (ACE2) with high affinity (KD = 15 nM). The S2 domain (aa 686-1213), which is reported to be well conserved, is responsible for membrane fusion. Over the course of the pandemic, SARS-CoV2 has consistently mutated giving rise to various mutants. The Alpha variant of SARS-CoV-2 contains 19 nonsynonymous mutations across its viral genome, including 8 substitutions or deletions in the spike protein. The N501Y mutation in Alpha variant results in enhanced viral replication in human airway epithelial cells and is a critical determinant of enhanced infection of the upper airway and transmission. This mutation resides in the ACE2 interface of the receptor binding domain (RBD). Clone 6D4-E2 specifically detects the SARS-CoV-2 Spike mutation N501Y. (Ref.: Liu, Y., et al. (2022). Nature. 602(7896); 294-299; Cheng, L., et al. (2021). Virol. J. 18; 87).

外觀

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

重構

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommended storage: +2°C to +8°C.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable