MABF2793

Anti-Smith Antigen Antibody, clone Y12

• 分類程式碼代碼: 12352203
• NACRES: NA.74

屬性

• 生物源: mouse
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: Y12, monoclonal
• 分子量: calculated mol wt 25 kDa; observed mol wt ~25 kDa
• 純化經由: using protein G
• 物種活性: human, mouse
• 包裝: antibody small pack of 100 μL
• 技術: immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunoprecipitation (IP): suitable; western blot: suitable
• 同型: IgG3κ
• UniProt登錄號: N/A
• 運輸包裝: 2-8°C
• 目標翻譯後修改: unmodified

描述

一般說明

Systemic lupus erythematosus (SLE) is a chronic autoimmune disease with a wide range of clinical manifestations and the presence of multiple autoantibodies. Anti-Smith antibodies are highly specific for SLE and are reported to be of importance in the classification of this disease. Smith antigens (Sm) are RNase resistant, non-histone nuclear proteins that are composed of several polypeptides of differing molecular weights. They include B, B , D1, D2, D3, E, F, and G polypeptides and associate with small ribonucleoproteins U1, U2, U4, U5, and U6. They are reported to be essential for the splicing of pre-mRNA in eukaryotes. Of these D1 and D3 are the most common antigens recognized by anti-Sm antibodies found in SLE subjects. Although Sm proteins are antigenically conserved and exist as RNA-protein complex, RNA does not play a major role in its antigenicity. Clone Y12 is reported to strongly react with native D1, D3, and B in immunoblot applications, but not with recombinantly expressed D1. It requires symmetrical dimethylarginines (sDMAs) for antigen recognition. This clone is reported to recognize an linear epitope (aa 95-119) in human D1 protein. (Ref.: Brahms, H., et al. (2000). J. Biol. Chem. 275(22); 17122-17129; Pettersson, I., et al. (1984). J. Biol. Chem. 259(9); 5907-5914; Dang, H., et al. (1983). J. Immunol 130(6); 2782-2785).

特異性

Clone Y12 is a mouse monoclonal antibody that detects Smith Antigen.

免疫原

Human Smith antigen.

應用

Quality Control Testing

Evaluated by Western Blotting in HCT116 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Smith Antigen in HCT116 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:500 dilution from a representative lot detected Smith Antigen in Caco-2 cell lysate.

Immunocytochemistry Analysis: A representative lot detected Smith Antigen in Immunocytochemistry (dos Santos, N.R. et al. (1997). Hum Mol Genet. 6(9): 1549-58).

Western Blotting Analysis: A representative lot detected Smith Antigen in Western Blotting applications (Naro, C. et al. (2019). Cell Rep. 26(11): 2929-2941; Somarelli, J.A., et al. (2011). Lupus. 20(3): 274-89; Brahms, H. et al. (2000). J Biol Chem. 275(22): 17122-9).

RNA Binding Protein Immunoprecipitation (RIP): A representative lot detected Smith Antigen in RNA Binding Protein Immunoprecipitation applications (Naro, C. et al. (2019). Cell Rep. 26(11):2929-2941).

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Smith Antigen in human tonsil tissue sections.

ELISA Analysis: A representative lot detected Smith Antigen in ELISA applications (Zhou, Y, et al. (2017). J Immunol. 198(5):1846-1854).

Immunohistochemistry Applications: A representative lot detected Smith Antigen in Immunohistochemistry applications (Mirra, A., et al. (2017). Sci Rep. 7(1):2033).

Immunoprecipitation Analysis: A representative lot immunoprecipitated Smith Antigen in Immunoprecipitation applications (Learner, E.A., et al. (1981). Proc Natl Acad Sci USA. 78(5):2737-41; Naro, C. et al. (2019). Cell Rep. 26(11): 2929-2941; Mirra, A., et al. (2017). Sci Rep.;7(1): 2033; Fischer, U. et al. (1997). Cell. 90(6):1023-9).

Radioimmunoassay: A representative lot detected Smith Antigen in Radioimmunoassay applications (Learner, E.A., et al. (1981). Proc Natl Acad Sci USA.;78(5):2737-41).

Immunofluorescence Analysis: A representative lot detected Smith Antigen in Immunofluorescence applications (Learner, E.A., et al. (1981). Proc Natl Acad Sci USA.78(5):2737-41; Mirra, A., et al. (2017). Sci Rep. 7(1):2033).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Smith Antigen, clone Y12, Cat. No. MABF2793, a mouse monoclonal antibody detects Smith antigen and is tested in ELISA, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, RIA, RNA Binding Protein IP (RIP), and Western Blotting.

外觀

Purified mouse monoclonal antibody IgG3 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable