MABF264

Anti-AIP1/WD repeat-containing protein 1, clone 13F10 Antibody

clone 13F10, from rat

• 同義詞: WD repeat-containing protein 1, Actin-interacting protein 1, AIP1, AIP1/WD repeat-containing protein 1
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rat
• 品質等級: 300
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: 13F10, monoclonal
• 物種活性: mouse
• 技術: western blot: suitable
• 同型: IgG2bκ
• NCBI登錄號: NP_035845
• UniProt登錄號: O88342
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: mouse ... Wdr1(22388)

描述

一般說明

Actin-interacting protein 1 (AIP1), also known as WD repeat-containing protein 1 (Wdr1), is a key player in cytoskeletal rearrangements and actin mobilization, and thus plays a role in many cellular situations from movement to cytokinesis. AIP1 interacts with Cofilin as well, and Cofilin regulates actin filament dynamics by stimulating actin filament disassembly and also plays a critical role in cytokinesis and chemotactic migration. Knockdown of AIP1 in HeLa cells increased the percentage of multinucleate cells and the mechanics of cytokinesis were altered with an excessive buildup of actin filaments at telophase. The results could be relieved by overexpression of Cofilin pointing to AIP1’s critical role in Cofilin-mediated actin filament disassembly. Furthermore, AIP1 inhibition caused abnormal cell migration in response to cytokine stimulation leading to a non-directional migration phenotype thus indicating that AIP1 plays an important directional role in response to stimuli as well. Finally AIP1, as one might expect, plays a critical role in development of cells and tissues, especially in epithelial morphogenesis for instance.

免疫原

Recombinant protein corresponding to mouse AIP1/WD repeat-containing protein 1.

應用

This Anti-AIP1/WD repeat-containing protein 1, clone 13F10 Antibody is validated for use in Western Blotting for the detection of AIP1/WD repeat-containing protein 1.

Western Blotting Analysis: A representative lot detected AIP1/WD repeat-containing protein 1 in both endogenous and transfected mouse AIP1 in western blots of cell lysates from J774 cells, 293T cells and 293T cells transfected with HA-tagged mouse Aip1 (Li, J., et al. (2007). Nature. 9(3):276-286).

品質

Evaluated by Western Blotting in RAW264.7 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected AIP1/WD repeat-containing protein 1 in 10 µg of RAW264.7 cell lysate.

標靶描述

~66 kDa observed

外觀

Format: Purified

其他說明

Concentration: Please refer to lot specific datasheet.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids