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Merck
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Key Documents

MABF2248

Sigma-Aldrich

Anti-EBV LMP1 Antibody, clone S12

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

抗體表格

purified antibody

無性繁殖

S12
monoclonal

純化經由

using protein G

物種活性

mouse, virus

濃度

(Please refer to lot specific datasheet.)

技術

flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

同型

IgG2aκ

UniProt登錄號

一般說明

A fusion protein (pKH548) consisting of 189 amino acids from the C-terminus region of LMP1 coupled to beta-galactosidase.

特異性

Clone S12 is a mouse monoclonal antibody that specifically detects Latent membrane protein 1 (LMP1) in Epstein-Barr virus transformed cells.

應用

Immunocytochemistry Analysis: A representative lot detected EBV LMP1 in Immunocytochemistry applications (Xu, J., et. al. (2002). J Virol. 76(8):4080-6).

Flow Cytometry Analysis: A representative lot detected EBV LMP1 in Flow Cytometry applications (Mann, K.P., et. al. (1985). J Virol. 55(3):710-20).

Western Blotting Analysis: A representative lot detected EBV LMP1 in Western Blotting applications (Choi, I.K., et. al. (2018). Proc Natl Acad Sci USA. 115(4):E686-E695; Young, L.S., et. al. (1988). J Gen Virol. 69 ( Pt 5):1051-65; Mann, K.P., et. al. (1985). J Virol. 55(3):710-20).

Immunofluorescence Analysis: A representative lot detected EBV LMP1 in Immunofluorescence applications (Mann, K.P., et. al. (1985). J Virol. 55(3):710-20).

Immunohistochemistry Analysis: A representative lot detected EBV LMP1 in Immunohistochemistry applications (Brink, A.A., et. al. (1997). J Clin Pathol. 50(11):911-8; Khabir, A., et. al. (2005). Virol J. 2:39).

Immunoprecipitation Analysis: A representative lot immunoprecipitated EBV LMP1 in Immunoprecipitation applications (Young, L.S., et. al. (1988). J Gen Virol. 69 ( Pt 5):1051-65).

品質

Evaluated by Western Blotting in Raji cell lysates.

Western Blotting Analysis: 1 µg/mL of this antibody detected LMP1 in lysates from Raji cells.

物理性質

~59 kDa observed; 41.98 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外觀

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Virus transformed human cells.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

分析證明 (COA)

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