MABE968

Anti-HLCS Antibody, clone 2C2.1

clone 2C2.1, from mouse

• 同義詞: Biotin--protein ligase, HLCS, Biotin apo-protein ligase, Biotin--[methylmalonyl-CoA-carboxytransferase] ligase, Biotin--[propionyl-CoA-carboxylase [ATP-hydrolyzing]] ligase, Holocarboxylase synthetase, HCS, Biotin--[methylcrotonoyl-CoA-carboxylase] ligas
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: 2C2.1, monoclonal
• 物種活性: human
• 技術: western blot: suitable
• 同型: IgG2aκ
• NCBI登錄號: NP_000402
• UniProt登錄號: P50747
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... HLCS(3141)

描述

一般說明

Biotin-protein ligase (EC 6.3.4.9; EC 6.3.4.10; EC 6.3.4.11; EC 6.3.4.15; UniProt P50747; also known as Biotin-[acetyl-CoA-carboxylase] ligase, Biotin apo-protein ligase, Biotin-[methylcrotonoyl-CoA-carboxylase] ligase, Biotin-[methylmalonyl-CoA-carboxytransferase] ligase, Biotin-[propionyl-CoA-carboxylase [ATP-hydrolyzing]] ligase, HCS, Holocarboxylase synthetase) is encoded by the HLCS (also known as HCS) gene (Gene ID 3141) in human. HLCS (holocarboxylase synthetase) is the sole biotin protein ligase encoded by the human genome. HLCS catalyses the covalent modification of target lysine residues by biotin. In addiition to taregting various carboxylases in the cytoplasm and mitochondria, both the full-length HLCS and a splicing variant are reported to enter the nucleus and associate with multi-protein gene repression complex. HLCS overexpression in HEK293 cells is reported to downregulate histone H3 lysine9 acetylation (H3K9ac) level with a cocomitant drop in LTRs (long terminal repeats) 15 and 22 due to a decrease in mRNA coding for LTRs. HLCS is also reported to induce RANTES expression in HEK-293 cells by biotinylating heat shock protein 72 (HSP72).

免疫原

Epitope: N-terminal region

GST-tagged recombinant protein corresponding to the N-terminal region of human HLCS.

應用

Anti-HLCS Antibody, clone 2C2.1 is an antibody against HLCS for use in Western Blotting.

Research Category
Signaling

Research Sub Category
Metabolic Hormones & Receptors

品質

Evaluated by Western Blotting in human skeletal muscle tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected HLCS in 10 µg of human skeletal muscle tissue lysate.

標靶描述

~80 kDa observed. Uncharacterized band(s) may appear in some lysates.

外觀

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable