MABE939

Anti-phospho Histone H3 (Ser10), clone 6G8B7 Antibody

clone 6G8B7, 1 mg/mL, from rat

• 同義詞: H3S10P, Histone H3 (phospho Ser10), H3 histone, family 3A, H3.3A, H3 histone, family 3B, H3.3B
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rat
• 品質等級: 100
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 6G8B7, monoclonal
• 物種活性: human
• 濃度: 1 mg/mL
• 技術: ELISA: suitable; immunocytochemistry: suitable; western blot: suitable
• 同型: IgG2aκ
• NCBI登錄號: NP_002098
• UniProt登錄號: P84243
• 運輸包裝: wet ice
• 目標翻譯後修改: phosphorylation (pSer10)
• 基因資訊: human ... H3C1(8350)

描述

一般說明

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3.3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.

免疫原

KLH-conjugated linear peptide corresponding to human Histone H3 (Ser10).

應用

Immunocytochemistry Analysis: A 1:5000 dilution from a representative lot detected Histone H3 (Ser10) in HeLa cells (Prof. Taro Tachibana, Osaka City University).
ELISA Analysis: A representative lot detected Histone H3 (Ser10) using differently phosphorylated and unphosphorylated peptides unmodified H3, H3 S10ph, and H3 S28ph (Prof. Taro Tachibana, Osaka City University).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

This Anti-phospho Histone H3 (Ser10), clone 6G8B7 Antibody is validated for use in Western Blotting and Immunocytochemistry and ELISA for the detection of phospho Histone H3 (Ser10).

品質

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Histone H3 (Ser10) in 10 µg of nocodazole treated HeLa cell lysates.

標靶描述

~17 kDa observed

聯結

Replaces: 04-1093

外觀

Protein G Purified

Format: Purified

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable