MABE912

Anti-Ikaros Antibody, clone 4E9

clone 4E9, from mouse

• 同義詞: DNA-binding protein Ikaros, Ikaros family zinc finger protein 1, Lymphoid transcription factor LyF-1
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 4E9, monoclonal
• 物種活性: human, mouse
• 技術: ChIP: suitable (ChIP-seq); flow cytometry: suitable; western blot: suitable
• 同型: IgG1κ
• NCBI登錄號: NP_001207694
• UniProt登錄號: Q13422
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... IKZF1(10320) ; mouse ... Ikzf1(22778)

描述

一般說明

Ikaros, also known as IKZF1, is a transcription factor that is important for the regulation of lymphopoiesis and also the development of the immune system. It has also been linked to adult and fetal erythroid differentiation in humans. Ikaros has been shown to induce local chromatin accessibility during CD4+ T cell differentiation and therefore, activating Th2 expression.

免疫原

Recombinant protein corresponding to Human Ikaros.

應用

Flow Cytometry Analsysis: 1 µg of a representative lot of this antibody detected Ikaros in Mouse thymocytes

Chromatin Immunoprecipitation (ChIP): A representative lot of this antibody as part of a blend Ikaros clones E9 and Ik14 (catalogue number MABE912 and MABE913 respectively) was demonstrated to perform in ChIP. See Zhang et al. (2012).

ChIP-seq Analsysis: A representative lot of this antibody as part of a blend Ikaros clones E9 and Ik14 (catalogue number MABE912 and MABE913 respectively) was demonstrated to perform in ChIP-seq. See Zhang et al. (2012).

Research Category
Epigenetics & Nuclear Function

Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Chromatin Biology

This Anti-Ikaros Antibody, clone 4E9 is validated for use in Western Blotting and Flow Cytometry and Chromatin Immunoprecipitation (ChIP) and ChIP-seq for the detection of Ikaros.

品質

Evaluated by Western Blotting in a Jurkat cell lysate.

Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected Ikaros in a Jurkat cell lysate.

標靶描述

~60 kDa observed. Uncharacterized bands may be observed in some lysates.

外觀

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable