MABE676

Anti-PHF1 Antibody, clone 12C2.1

clone 12C2.1, from mouse

• 同義詞: PHD finger protein 1, hPHF1, Polycomb-like protein 1, hPCl1
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: 12C2.1, monoclonal
• 物種活性: human, mouse
• 技術: immunohistochemistry: suitable (paraffin); western blot: suitable
• 同型: IgG2bκ
• NCBI登錄號: NP_002627
• UniProt登錄號: O43189
• 運輸包裝: ambient
• 目標翻譯後修改: unmodified
• 基因資訊: human ... PHF1(5252)

描述

一般說明

PHD finger protein 1 (UniProt: O43189; also known as PHF1, hPHF1, Polycomb-like protein 1, hPCl1) is encoded by the PHF1 (also known as PCL1) gene (Gene ID: 5252) in human. PHF1 is a member of the polycomblike family and contains two PHD-type zinc fingers (aa 87-142 and 186-240) and one Tudor domain (aa 29-86). It is essential for epigenetic regulation and genome maintenance. It specifically binds histone H3 trimethylated at ′Lys-36′ (H3K36me3) via its Tudor domain and recruits the PRC2 complex. PHF1 is highly expressed in heart, skeletal muscle, and pancreas, with lower levels found in brain, placenta, lung, liver and kidney. It is involved in DNA damage response and is recruited at double-strand breaks. PHF1 localizes to the promoters of numerous target genes and to double-strand breaks (DSBs) sites following DNA damage. It has been reported that PRC2 recruitment by PHF1 promotes H3K27me3 and subsequent gene silencing by inducing spreading of PRC2 and H3K27me3 into H3K36me3 loci. A chromosomal aberration involving PHF1 is reported to be the causative factor in endometrial stromal tumors. (Ref.: Sarma, K et al. (2008). Mol. Cell. Biol. 28(8): 2718-31; Musselman, CA et al (2012). Nat. Struct. Mol. Biol. 19(12):1266-72).

特異性

Clone 12C2.1 specifically detects PHF1 in human tissues. It targets an epitope within 137 amino acids from the internal region.

免疫原

GST/His-tagged recombinat fragment corresponding to 137 amino acids from human PHD finger protein 1.

應用

Anti-PHF1, clone 12C2.1, Cat. No. MABE676, is a highly specific mouse monoclonal antibody that targets PHF1 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected PHF1 in human esophagus and human tonsil tissue.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PHF1 in 10 µg of HeLa cell lysate.

Research Category
Epigenetics & Nuclear Function

品質

Evaluated by Western Blotting in mouse heart tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PHF1 in 10 µg of mouse heart tissue lysate.

標靶描述

~63 kDa observed; 62.11 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外觀

Format: Purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1