MABE1935

Anti-Sox-11 Antibody, clone SOX11-C1

• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: SOX11-C1, monoclonal
• 分子量: calculated mol wt 46.68 kDa
• 純化經由: using protein G
• 物種活性: human
• 包裝: antibody small pack of 100 μg
• 技術: flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• 同型: IgG1κ
• 表位序列: Internal
• Protein ID登錄號: NP_003099
• UniProt登錄號: P35716
• 運輸包裝: 2-8°C
• 目標翻譯後修改: unmodified

描述

一般說明

Transcription factor SOX-11 (UniProt: P35716; also known as SOX-11) is encoded by the SOX11 gene (Gene ID: 6664) in human. Sox-11 is a neural transcription factor that is involved in the embryonic neurogenesis and plays a role in tissue modeling during development, neuronal cell proliferation, and survival. Its expression is primarily observed in the brain and heart and low expression is detected in the kidney, pancreas, and muscle. Its transient activity during neurogenesis is critical to ensure the precise execution of the neurogenic program. Sox-11 serves as a transcription factor and acts as a transcriptional activator. It binds cooperatively with Brn2 or Oct6, which enhances its transcriptional activation. Sox-11 activity is controlled by epigenetics and transcriptional mechanisms and by post-translational modifications. Its three highly conserved phosphorylation sites, serine 30, 133, and 137, surrounding the DNA-binding high-mobility group (HMG box), are reported to modulate its transcriptional activity. These sites are unique to Sox-11. Serine 30 phosphorylation is reported to promote its nuclear over cytoplasmic localization. Phosphorylation of serine 133 by PKA modulates dendrite development of adult-born dentate granule neurons and fine tunes the activated of selected target genes. Higher expression of Sox-11 has been shown in Mantle cell lymphoma. Mutations in SOX11 gene are known to cause Coffin-Siris syndrome 9 that is characterized multiple malformations and intellectual disability. Mice deficient in Sox-11 exhibit defects in multiple organs including the lung, heart, and pancreas.

特異性

Clone SOX11-C1 is a mouse monoclonal antibody that detects human Sox-11. It targets an epitope within the internal region.

免疫原

His-tagged recombinant fragment corresponding to 121 amino acids from the internal region of human Sox-11.

應用

Quality Control Testing

Evaluated by Immunocytochemistry in SH-SY5Y cells.

Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected Sox-11 in SH-SY5Y cells.

Tested Applications

Flow Cytometry Analysis: A representative lot detected Sox-11 in Flow Cytometry applications (Nordstrom, L., et al. (2012). BMC Cancer.;12:269; Lu, T.X., et al. (2013). Leuk. Res. 37(11):1412-9).

Western Blotting Analysis: A representative lot detected Sox-11 in Western Blotting applications (Nordstrom, L., et al. (2012). BMC Cancer. 12:269).

Immunocytochemistry Analysis: A representative lot detected Sox-11 in Immunocytochemistry applications (Nordstrom, L., et al. (2012). BMC Cancer. 12:269).

Immunohistochemistry (Paraffin) Analysis: A representative lot detected Sox-11 in Immunohistochemistry applications (Nordstrom, L., et al. (2012). BMC Cancer. 12:269; Nordstrom, L., et al. (2014). Br. J. Haematol. 166(1):98-108; Lu, T.X., et al. (2013). Leuk Res. 37(11):1412-9).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Sox-11, clone SOX11-C1, Cat. No. MABE1935, is a mouse monoclonal antibody that detects SOX-11 and is tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

外觀

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable