MABE1934

Anti-IKFZ3 degron tag Antibody, clone 1C3-D3-F8

• 分類程式碼代碼: 12352203

屬性

• 生物源: mouse
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 1C3-D3-F8, monoclonal
• 分子量: calculated mol wt 58.02 kDa; observed mol wt ~39 kDa
• 純化經由: using protein G
• 物種活性: human
• 物種活性（以同源性預測）: monkey, bovine, rat
• 包裝: antibody small pack of 100 μL
• 技術: western blot: suitable
• 同型: IgG1κ
• 表位序列: N-terminal half
• Protein ID登錄號: NP_001244337
• UniProt登錄號: Q9UKT9
• 運輸包裝: 2-8°C
• 目標翻譯後修改: unmodified

描述

一般說明

Zinc finger protein Aiolos (UniProt: Q9UKT9; also known as Ikaros family zinc finger protein 3, IKZF3) is encoded by the IKZF3 (also known as ZNFN1A3) gene (Gene ID: 22806) in human. IKZF3 is a member of the Ikaros Zinc finger family of transcription factors with six C2H2 type zinc finger domains. It is expressed mainly in peripheral blood leukocytes, the spleen, and the thymus. It is present as a homodimer and can also form heterodimers with other Ikaros family members. It plays an important role in the regulation of lymphocyte differentiation and is involved in the regulation of B-cell differentiation, proliferation and maturation. It is also shown to regulate Bcl-2 expression and controls apoptosis in T-cells in an IL2-dependent manner. Its levels are shown to be upregulated by TGF-1. IKZF3 expression Th17 cells is associated with increased IL-10 production and it is shown to interact with known regulators of IL10 expression. It also acts as a transcriptional activator in CD4+ T cells in cooperation with other factors, such as FOXP3 and BLIMP1 in CD4+ regulatory T cells (Tregs). The degron sequence of IKZF3 is localized to amino acids 144-168 and a Q147H mutation is reported to cripple the function of IKZF3. This sequence constitutes a modular degron that can be used to target heterologous proteins. This degron sequence is as capable as the full-length IKZF3 at targeting selected proteins for degradation. It is shown to target green fluorescent protein (GFP) for pomalidomide-induced degradation when fused to the C-terminus of GFP, but not to the N-terminus. It has also been sown to target exogenous HIF2 for pomalidomide-induced degradation when fused to the C-terminus of HIF2 . Clone 1C3-D3-F8 recognizes the degron sequence that is considered as an important regulator of protein degradation. (Ref.: Koduri, V. et al. (2019). Proc. Natl. Acad. Sci. USA. 116(7); 2539-2544).

特異性

Clone 1C3-D3-F8 is a mouse monoclonal antibody that detects IKZF3 Protein. It targets an epitope within 25 amino acids from a Degron sequence in the N-terminal half.

免疫原

KLH-conjugated linear peptide corresponding to Degron sequence from human IKZF3.

應用

Quality Control Testing

Evaluated by Western Blotting in lysate from HEK293 cells expressing IKZF3 Degron-tagged PTPA.

Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected Zinc finger protein Aiolos (IKZF3) in lysates from HEK293 cells ectopically expressing IKZF3 Degron-tagged PTPA, but not in lysate from untransfected HEK293 cells (wild type).

Anti-IKFZ3 degron tag, clone 1C3-D3-F8, Cat. No. MABE1934, is a mouse monoclonal antibody that detects Zinc finger protein Aiolos (IKZF3) and is tested for use in Western Blotting.

外觀

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1