MABE132

Anti-MyoD Antibody, clone 5F11

clone 5F11, from rat

• 同義詞: Myoblast determination protein 1
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rat
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: 5F11, monoclonal
• 物種活性: mouse
• 技術: immunocytochemistry: suitable; western blot: suitable
• 同型: IgG2aκ
• NCBI登錄號: NP_034996
• UniProt登錄號: P10085
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: mouse ... Myod1(17927)

描述

一般說明

Myoblast determination protein 1 (MyoD1) is a myogenic transcription factor required for the growth, repair, and regeneration of muscle cells. It induces differentiation of myoblast cells into multinucleated myotubules, activating genes and proteins involved in cell cycle arrest, and differentiation. MyoD1 may also control proliferation and homeostasis during embryogenesis, in the absence of Myf5.

免疫原

GST-tagged recombinant protein corresponding to mouse MyoD.

應用

Detect MyoD using this Anti-MyoD Antibody, clone 5F11 validated for use in Western Blotting, ICC.

Immunocytochemistry Analysis: A representative lot detected MyoD in C2C12 cells.

Western Blot Analysis: A representative lot detected MyoD in C2C12 cells.

Research Category
Signaling

Research Sub Category
Developmental Signaling

品質

Evaluated by Western Blot in C2C12 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected MyoD in 10 µg of C2C12 cell lysate.

標靶描述

~45 kDa observed. An uncharacterized band at ~38 kDa may be observed in some cell lysates. The calculated molecular weight is 34 kDa; however, this protein is known to be glycosylated.

聯結

Replaces: MABE514

外觀

Protein G Purified

Format: Purified

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

分析報告

Control
C2C12 cell lysate

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable