MABE1039

Anti-Nup96 Antibody, clone 4H5

clone 4H5, from rat

• 同義詞: Nuclear pore complex protein Nup98-Nup96, Nuclear pore complex protein Nup98, 98 kDa nucleoporin, Nucleoporin Nup98, Nup98, Nuclear pore complex protein Nup96, 96 kDa nucleoporin, Nucleoporin Nup96, Nup96, Nup96
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702

屬性

• 生物源: rat
• 品質等級: 100
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 4H5, monoclonal
• 物種活性: mouse
• 技術: immunocytochemistry: suitable; western blot: suitable
• 同型: IgG2aκ
• NCBI登錄號: NP_001274093
• UniProt登錄號: Q6PFD9
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: mouse ... Nup98(269966)

描述

一般說明

The murine gene Nup98 (Gene ID 269966; also known as AI849286, 4732457F17) encodes the 1816-amino acid nuclear pore complex protein Nup98-Nup96 (UniProt Q6PFD9), which is posttranslationally autocleaved between aa 800 and 801 to form Nup98 (aa 1-800) and Nup96 (aa 801-1816). The nuclear pore complex (NPC) acts as a passageway for nucleocytoplasmic transport. The core structure of the NPC is maintained by the Nup93-205 complex sandwiched between the supporting structures of the Nup107-160 complex on the nuclear and cytoplasmic faces of the pore. Nup96 is a component of the Nup107-160 complex, the largest subunit of the NPC. Nup96 knockout is reported to be fatal in mice and Nup96(+/-) mice with low levels of Nup96 display impaired immune response and are highly susceptible to viral infection.

特異性

Reacts with Nup98-Nup96 precursor (isoforms 1, 2, and 6), nucleoporin Nup96 (isoform 5), but not nucleoporin Nup98 (isoform 3).

免疫原

Epitope: Near N-terminus.

GST-tagged recombinant protein corresponding to mouse Nup96 near the N-terminus.

應用

Anti-Nup96 Antibody, clone 4H5 is an antibody against Nup96 for use in Western Blotting and Immunocytochemistry.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

Western Blotting Analysis: A representative lot detected Nup96 in NIH/3T3 cells (Mizuguchi, C., et al. (2010). Hybridoma. 29(6):551-553).
Immunocytochemistry Analysis: A representative lot detected Nup96 in NIH/3T3 cells (Mizuguchi, C., et al. (2010). Hybridoma. 29(6):551-553).

品質

Evaluated by Western Blotting in NIH/3T3 cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected Nup96 in 10 µg of NIH/3T3 cell lysate.

標靶描述

~120 kDa observed

外觀

Protein G Purified

Format: Purified

Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable