跳轉至內容
Merck
全部照片(1)

Key Documents

MABE1015

Sigma-Aldrich

Anti-CST complex subunit CTC1 Antibody, clone 8E5.2

clone 8E5.2, from mouse

同義詞:

CST complex subunit CTC1, Conserved telomere maintenance component 1, HBV DNAPTP1-transactivated protein B

登入查看組織和合約定價


About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

mouse

品質等級

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

8E5.2, monoclonal

物種活性

human

技術

western blot: suitable

同型

IgG1κ

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... CTC1(80169)

一般說明

CST complex subunit CTC1 (UniProt Q2NKJ3; also known as Conserved telomere maintenance component 1, HBV DNAPTP1-transactivated protein B) is encoded by the CTC1 (also known as C17orf68, CRMCC) gene (Gene ID 80169) in human. CTC1 is a subunit of the trimeric CST (CTC1, STN1, and TEN1) complex that binds ssDNA, promotes telomere DNA synthesis, and inhibits telomerase-mediated telomere elongation. CST directly interacts with and stimulates DNA polymerase α-primase (polα-primase) activity. CST also associates with and sequesters telomeric 3′ overhangs after their elongation by telomerase, thereby limiting further telomere extension by telomerase. Knockdown of either STN1 or CCT1 in human cancer cells and in mouse fibroblasts results in substantially elongated telomeric G-overhangs. Conditional deletion of CTC1 in mice leads to early entry into senescence and signs of telomeric replication defects, Human CTC1 gene mutations casue dyskeratosis congenita (DC) and related bone marrow failure syndromes, as well as the rare autosomal recessive neurological disorder Coats Plus, where patients develop cranial calcifications and cysts. Coats Plus and DC patients develop premature hair graying, anemia, and osteoporosis as a result of telomerase defects.

特異性

Expected to react with both spliced isoforms of human CTC1 reported by UniProt (Q2NKJ3).

免疫原

Epitope: Internal (C-terminal half).
GST-tagged recombinant internal fragment from the C-terminal half of human CTC1.

應用

Anti-CST complex subunit CTC1 Antibody, clone 8E5.2 is an antibody against CST complex subunit CTC1 for use in Western Blotting.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair

品質

Evaluated by Western Blotting in human cortex tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected CST complex subunit CTC1 in 10 µg of human cortex tissue lysate.

標靶描述

~135 kDa observed. 134.6 kDa (isoform 1) and 114.1 kDa (isoform 2) calculated. Uncharacterized band(s) may appear in some lysates.

外觀

Protein G Purified
Format: Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

未找到適合的產品?  

試用我們的產品選擇工具.

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。

已經擁有該產品?

您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.

聯絡技術服務