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一般說明
Antigen-presenting glycoprotein CD1d (UniProt P15813; also known as CD1d, R3G1) is encoded by the CD1D gene (Gene ID 912) in human. CD1d is an MHC-like glycoprotein expressed on epithelial cells and can signal innate immune responses by presenting lipid antigen to natural killer (NK) and NK T (NKT) cells. Similar to MHC class I heavy chain (HC), CD1d HC is glycosylated and assembled with β2m in ER. Unlike MHC class I HC, misfoled CD1d and CD1d not properly assembled with β2m are not destroyed by ER quality control mechanisms. CD1d plays a role in both innate and adaptive immunity to various bacteria, viruses, fungi, and parasites. Activation of CD1d-restricted invariant NKT cells enhances host resistance to these microbes via direct killing of CD1d-expressing (infected) cells or by promoting interferon gamma release from conventional NK cells and by modulating adaptive immune cells via alteration of Th1/Th2 polarization. Chlamydia trachomatis is shown to prevent CD1d-mediated innate immune destruction of the infected urethral epithelial cells by targeting CD1d for proteolytic degradation. Specifically, the chlamydial proteasome-like activity factor (CPAF) interacts with the CD1d HC, and CPAF-associated CD1d HC is then ubiquitinated for eventual degradation.
免疫原
Epitope: CD1d α1 domain
Pooled CD1d-GST fusion proteins containing α1 domain alone, α1-α2 domains, or α1–α3 domains.
應用
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
This Anti-CD1d Antibody, clone D5 is validated for use in Western Blotting, Immunoprecipitation for the detection of CD1d.
Western Blotting Analysis: A representative lot detected time-dependent CD1d degradation in CD1d immunoprecipitate from human penile urethral epithelial (PURL) cells following C. trachomatis infection (Kawana, K., et al. (2007). J Biol Chem. 282(10):7368-7375).
Western Blotting Analysis: A representative lot detected exogenously expressed CD1d in CD1d-transfected C1R human lymphoblastoid cells (Kim, H.S., et al. (1999). J Biol Chem. 274(14):9289-9295).
Immunoprecipitation Analysis: A representative lot immunoprecipitated intact as well as degraded CD1d from human penile urethral epithelial (PURL) cells following C. trachomatis (Kawana, K., et al. (2007). J Biol Chem. 282(10):7368-7375).
Immunoprecipitation Analysis: A representative lot immunoprecipitated exogenously expressed CD1d independent of glycosylation states from lysates of CD1d-transfected FO-1 human melanoma cells (Kim, H.S., et al. (1999). J Biol Chem. 274(14):9289-9295).
Western Blotting Analysis: A representative lot detected exogenously expressed CD1d in CD1d-transfected C1R human lymphoblastoid cells (Kim, H.S., et al. (1999). J Biol Chem. 274(14):9289-9295).
Immunoprecipitation Analysis: A representative lot immunoprecipitated intact as well as degraded CD1d from human penile urethral epithelial (PURL) cells following C. trachomatis (Kawana, K., et al. (2007). J Biol Chem. 282(10):7368-7375).
Immunoprecipitation Analysis: A representative lot immunoprecipitated exogenously expressed CD1d independent of glycosylation states from lysates of CD1d-transfected FO-1 human melanoma cells (Kim, H.S., et al. (1999). J Biol Chem. 274(14):9289-9295).
品質
Evaluated by Western Blotting in THP-1 cell lysate.
Western Blotting Analysis: 4.0 µg/mL of this antibody detected CD1d in 10 µg of THP-1 cell lysate.
Note: Longer exposure time is needed when using total lysates for Western blotting applications. Membrane preparation or immunoprecipitation can be employed to enrich membrane target proteins prior to Western blotting.
Western Blotting Analysis: 4.0 µg/mL of this antibody detected CD1d in 10 µg of THP-1 cell lysate.
Note: Longer exposure time is needed when using total lysates for Western blotting applications. Membrane preparation or immunoprecipitation can be employed to enrich membrane target proteins prior to Western blotting.
標靶描述
~37/43 kDa observed
外觀
Protein G Purified
Format: Purified
Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
儲存和穩定性
Stable for 1 year at 2-8°C from date of receipt.
其他說明
Concentration: Please refer to lot specific datasheet.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
CD1d degradation in Chlamydia trachomatis-infected epithelial cells is the result of both cellular and chlamydial proteasomal activity.
Kawana, K; Quayle, AJ; Ficarra, M; Ibana, JA; Shen, L; Kawana, Y; Yang, H; Marrero et al.
The Journal of Biological Chemistry null
Biochemical characterization of CD1d expression in the absence of beta2-microglobulin.
Kim, HS; Garcia, J; Exley, M; Johnson, KW; Balk, SP; Blumberg, RS
The Journal of Biological Chemistry null
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