MABC969

Anti-PD-L2 Antibody, clone 24F.10C12

clone 24F.10C12, from mouse

• 同義詞: Programmed cell death 1 ligand 2, B7-DC, B7 dendritic cell molecule, B7-H2, Butyrophilin B7-DC, CD273, PD-1 ligand 2, PD-L2, PDCD1 ligand 2, Programmed death ligand 2
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: 24F.10C12, monoclonal
• 物種活性: human
• 技術: flow cytometry: suitable; immunohistochemistry: suitable; neutralization: suitable; western blot: suitable
• 同型: IgG2aκ
• NCBI登錄號: NP_079515.2
• UniProt登錄號: Q9BQ51
• 運輸包裝: dry ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... PDCD1LG2(80380)

描述

一般說明

Programmed cell death 1 ligand 2 (UniProt Q9BQ51; also known as B7-DC, B7 dendritic cell molecule, B7-H2, Butyrophilin B7-DC, CD273, PD-1 ligand 2, PD-L2, PDCD1 ligand 2, Programmed death ligand 2) is encoded by the PDCD1LG2 (also known as B7DC, CD273, PDCD1L2, PDL2) gene (Gene ID 80380) in human. PD-1 and PD-1 ligands 1&2 (PD-L1 and PD-L2) are B7:CD28 family members that regulate T cell activation and peripheral tolerance. When engaged together with the TCR, the interaction of PD-1 with its ligands delivers an inhibitory signal to T cell proliferation and cytokine production. While PD-L1 is broadly expressed in hematopoietic and nonhematopoietic cells, PD-L2 expression is highly restricted to antigen presenting cells (APCs), including dendritic cells (DCs) and macrophages. The PD-1 pathway plays a key role in the progressive loss of effector T cell responses during chronic HIV infection. Under some conditions, blockade of this pathway is able to restore many T cell functions. PD-L2 is initially produced with signal peptide (a.a. 1-19) sequence, the removal of which yields the mature protein with a large extracellular (a.a. 20-220) region that contains an Ig-like V-type domain (a.a. 21-118) and an Ig-like C2-type domain (a.a. 122-203), followed by a transmembrane domain (a.a. 221-241) and a cytoplasmic tail (a.a. 242-273).

特異性

Clone 24F.10C12 immunostained the surface of 300.19 murine pre-B lymphoma cells transfected with human PD-L2, but not the untransfected cells or cells transfected with human PD-L1 (Brown, J.A., et al. (2003). J. Immunol. 170(3):1257-1266). Reactivity toward spliced isoform 2 (PD-L2II) and isoform 3 (PD-L2III) has not been determined.

免疫原

Epitope: Extracellular domain.

Recombinant human PD-L2.

應用

Flow Cytometry Analysis: 0.1 µg from a representative lot detected PD-L2-positive human PBMCs.
Flow Cytometry Analysis: A representative lot immunostained the surface of 300.19 murine pre-B lymphoma cells transfected with human PD-L2, but not the untransfected cells or cells transfected with human PD-L1 (Brown, J.A., et al. (2003). J. Immunol. 170(3):1257-1266).
Flow Cytometry Analysis: A representative lot was conjugated with FITC and detected a small induction of surface PD-L2 expression on human PBMCs in culture upon IFN-gamma stimulation (Brown, J.A., et al. (2003). J. Immunol. 170(3):1257-1266).
Flow Cytometry Analysis: A representative lot was conjugated with FITC and employed to detect surface PD-L2 expression on PBMC-derived dendric cells (DCs). An upregulated PD-L2 expression was seen among mature DCs (mDC) than immature DCs (iDC), the levels of PD-L2 were reduced upon pretreatment of mDC with IL-10 (Brown, J.A., et al. (2003). J. Immunol. 170(3):1257-1266).
Flow Cytometry Analysis: A representative lot was conjugated with FITC and detected surface PD-L2 expression on tonsil-derived human follicular DCs (FDCs) (Brown, J.A., et al. (2003). J. Immunol. 170(3):1257-1266).
Neutralizing Analysis: A representative lot competed against human PD-1 extracellular domain Ig fusion for the binding of exogenously expressed human PD-L2 on the surface of 300.19 murine pre-B lymphoma cells (Brown, J.A., et al. (2003). J. Immunol. 170(3):1257-1266).
Neutralizing Analysis: DP-L2 blocking on the surface of PBMC-derived dendritic cells (DCs) by clone 24F.10C12 Fab fragment increased CD4+ T cell proliferation and cytokine production in allogenic cultures with immature DCs (iDC), mature DCs (mDC), and IL-10-treated mDCs. Dual blockage of both DP-L1 (by clone 29E.2A3 Fab fragment) and DP-L2 synergized the enhancing effect (Brown, J.A., et al. (2003). J. Immunol. 170(3):1257-1266).
Immunohistsochemistry Analysis: A representative lot detected PD-L2 expression pattern in frozen human tonsil, placenta, fetal thymus and cardiac tissue sections (Brown, J.A., et al. (2003). J. Immunol. 170(3):1257-1266).
Immunocytsochemistry Analysis: A representative lot detected an upregulated PD-L2 immunoreactivity in human CD14+ monocyte-derived macrophages (MDMs) following HIV-BaL infection, but not LPS stimulation by fluorescent immunocytochemistry using paraformaldehyde-fixed MDMs (Rodríguez-García, M., et al. (2011). J. Leukoc. Biol. 89(4) 507–515).

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-PD-L2 Antibody, clone 24F.10C12 is validated for use in Western Blotting, Flow Cytometry, Neutralizing, Immunohistochemistry for the detection of PD-L2.

品質

Evaluated by Western Blotting in human thymus tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected PD-L2 in 10 µg of human thymus tissue lysate.

標靶描述

~30 kDa observed. 28.85 kDa (isoform 1; PD-L2I), 18.76 kDa (isoform 2; PD-L2II), 18.64 kDa (isoform 3; PD-L2III) calculated.

外觀

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2aκ antibody in buffer containing PBS without preservatives.

儲存和穩定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable