MABC612

Anti-Procadherin FAT1 Antibody, clone C257

clone C257, from mouse

• 同義詞: Cadherin family member 7, Cadherin-related tumor suppressor homolog, Protein fat homolog
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: C257, monoclonal
• 物種活性: human
• 技術: immunoprecipitation (IP): suitable; western blot: suitable
• 同型: IgG1κ
• NCBI登錄號: NP_005236
• UniProt登錄號: Q14517
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... FAT1(2195)

描述

一般說明

Procadherin FAT1 protein is a cell membrane bound protein that also has an active nuclear-translocation component that can alter specific gene expression and cell proliferation. Procadherin FAT1 is important in cell polarization and directed cell migration and also modulates cell-cell contact interactions. Procadherin FAT1 is expressed in epithelial, endothelial and smooth muscle cells and plays roles in their response to injury and is often highly induced after arterial injury and effects vascular smooth muscle cell growth and migration. Procadherin FAT1 is also important in cancer where it appears to function as both an oncogene and a tumor suppressor depending upon the cell context. Also recent research has shown that soluble FAT1 can be a novel biomarker candidate for such cancers as pancreatic cancer.

免疫原

Recombinant protein corresponding to Human FAT1.

應用

Research Category
Apoptosis & Cancer

This Anti-Procadherin FAT1 Antibody, clone C257 is validated for use in Western Blotting and Immunoprecipitation for the detection of Procadherin FAT1.

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Procadherin FAT1 in 10 µg of FAT1 transfected MV3 cell lysate.
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Procadherin FAT1 in 10 µg of FAT1 transfected MDA-MB-231 and MDA-MB-468 cell lysate.
Immunoprecipitation Analysis: A representative lot immunoprecipitated Procadherin FAT1 in C257 cell lysate. (Courtesy of Dr. R. Thorne, U of Newcastle, AU.)

品質

Evaluated by Western Blotting in FAT1 transfected NIH-3T3 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Procadherin FAT1 in 10 µg of FAT1 transfected NIH-3T3 cell lysate.

標靶描述

>260 kDa observed. Procadherin FAT1 is an extremely large cell surface receptor with a calculated MW of 506 kDa and it′s highly glycosolated. It may be difficult to resolve the protein by western blot. An uncharacterized band at 50 kDa and other lower MWs may be observed in some lysates.

外觀

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable