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重要文件

MABC1166

Sigma-Aldrich

Anti-iASPP Antibody, clone LX128.5

clone LX128.5, from mouse

同義詞:

RelA-associated inhibitor, Inhibitor of apoptosis-stimulating protein of p53, Inhibitor of ASPP protein, NF-kB-interacting protein 1, PPP1R13B-like protein, Protein iASPP

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

mouse

品質等級

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

LX128.5, monoclonal

物種活性

mouse

物種活性(以同源性預測)

human (based on 100% sequence homology)

技術

immunohistochemistry: suitable (paraffin)

同型

IgG1κ

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

一般說明

RelA-associated inhibitor (UniProt Q8WUF5; also known as Inhibitor of apoptosis-stimulating protein of p53, Inhibitor of ASPP protein, NF-kB-interacting protein 1, PPP1R13B-like protein, Protein iASPP) is encoded by the PPP1R13L (also known as IASPP, NKIP1, PPP1R13BL, RAI) gene (Gene ID 10848) in human. Inhibitor of apoptosis-stimulating protein of p53 (iASPP) belongs to the ASPP family of proteins (ASPP1, ASPP2, and iASPP) characterized by their ankyrin repeats (a.a. 659-691 & 692-724 in iASPP), SH3 domain (a.a. 758-820 in iASPP), and proline-rich (a.a. 54-602 in iASPP) sequences. iASPP is expressed at intercalated discs in post-mitotic cardiomyocytes, where it interacts with desmoplakin and desmin in cardiomyocytes to help maintain the integrity of desmosomes and intermediate filament networks. Desmosomes are anchoring junctions found in cells that endure physical stress such as cardiac myocytes or skin keratinocytes. Mutations of genes coding desmosome components, including IASPP/PPP1R13L, are found in human patients suffering from cardiomyopathy and in animal models of cardiomyopathy. iASPP deficiency caused by spontaneous mutation has been reported to be linked to a lethal autosomal recessive cardiomyopathy in Poll Hereford calves and Wa3 mice. iASSP-deficient mice with exon 8 deletion display features of arrhythmogenic right ventricular cardiomyopathy (ARVC) and die of sudden cardiac death.

特異性

Clone LX128.5 immunostained intercalated discs in myocardial sections from wild-type, but not iASPP-deficient (iASPPΔ8/Δ8) adult mice (Notari, M., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(9):E973-E981).

免疫原

Synthetic linear peptide corresponding to a sequence from the N-terminal region of human iASPP.

應用

Anti-iASPP Antibody, clone LX128.5 is an antibody against iASPP for use in Immunohistochemistry (Paraffin).
Immunohistochemistry Analysis: A representative lot immunostained intercalated discs in myocardial sections from adult wild-type, but not iASPP-deficient (iASPPΔ8/Δ8) mice (Notari, M., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(9):E973-E981).
Note: iASPP is expressed at intercalated discs. To prepare heart tissue sections for iASPP staining, it is important to cut the tissues at an angle parallel to the plane of the intercalated discs.

品質

Evaluated by Immunohistochemistry in mouse heart tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected iASPP in mouse heart tissue.

標靶描述

89.09 kDa calculated.

外觀

Format: Purified

其他說明

Concentration: Please refer to lot specific datasheet.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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