MAB5254

Anti-Neurofilament 160 kDa Antibody, clone NN18

clone NN18, Chemicon^®, from mouse

• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: NN18, monoclonal
• 物種活性: guinea pig, chicken, rat, mouse, pig
• 物種活性（以同源性預測）: human
• 製造商／商標名: Chemicon^®
• 技術: immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• 同型: IgG1
• NCBI登錄號: NM_001105541.1
• UniProt登錄號: P07197
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: chicken ... Nefm(396206) ; human ... NEFM(4741) ; mouse ... Nefm(18040) ; rat ... Nefm(24588)

描述

一般說明

Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information see Nervous System Cell Type Specific Marker chart online under the CHEMICON Technical Support section.

特異性

The antibody reacts with neurofilament 160 kD (Debus, E., 1982; Shaw, G., 1984).

免疫原

Purified neurofilament polypeptides.

應用

Anti-Neurofilament 160 kDa Antibody, clone NN18 is an antibody against Neurofilament 160 kDa for use in IH, IH(P) & WB.

Immunohistochemistry:
5-10 µg/mL on frozen or paraffin embedded tissue. See protocols for details.

Western Blot:
1:1000. 7.5%-10% SDS-PAGE

Optimal working dilutions and protocols must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Neurofilament & Neuron Metabolism

Neuronal & Glial Markers

品質

Routinely evaluated by Western Blot on PC12 lysates.

Western Blot Analysis:
1:500 dilution of this lot detected Neurofilament on 10 μg of PC12 lysates.

標靶描述

160 kDa

聯結

Replaces: CBL214

外觀

Protein A purified

Format: Purified

Purified mouse monoclonal IgG1 in buffer containing 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.

儲存和穩定性

Stable for 6 months at 2-8ºC in undiluted aliquots from date of receipt.
Do not freeze.

分析報告

Control
Brain

Cultured neurons

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable