MAB374-AF488

Anti-GAPDH Antibody, clone 6C5, Alexa Fluor^™ 488 conjugate

clone 6C5, from mouse, ALEXA FLUOR^™ 488

• 同義詞: Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH, GAPDH
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 共軛: ALEXA FLUOR^™ 488
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 6C5, monoclonal
• 物種活性: mouse, human
• 物種活性（以同源性預測）: cat (based on 100% sequence homology), fish (based on 100% sequence homology), canine (based on 100% sequence homology), porcine (based on 100% sequence homology), rat (based on 100% sequence homology), rabbit (based on 100% sequence homology)
• 技術: immunocytochemistry: suitable
• 同型: IgG1
• NCBI登錄號: NM_001082253.1
• UniProt登錄號: P46406
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... GAPDH(2597) ; mouse ... Gapdh(14433) ; rabbit ... Gapdh(100009074)

描述

一般說明

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an ubiquitous glycolytic enzyme present in reasonably high levels in almost all tissues. The complete and functional enzyme is a tetramer with each of four identical subunits occupying the vertex of a tetrahedron. Its binding domains also include one principally interacting with NAD+ and another interacting with glyceraldehyde 3-phosphate (GAP). As a ′house-keeping′ enzyme, it catalyzes the synthesis of 1,3-biphosphoglycerate, a "high energy" intermediate used for the synthesis of ATP. Besides its cytoplasmic role in metabolism, it is also involved in the initial stages of apoptosis or oxidative stress response where it is translocated to the nucleus. Such actions may reflect the role of GAPDH in DNA repair or as one nuclear carrier for apoptotic molecules. GAPDH can also bind specifically to proteins implicated in the pathogenesis of a variety of neurodegenerative disorders, including the beta-amyloid precursor protein and the Huntingtin protein where reduced function of GAPDH is associated with Alzheimer′s and Huntington′s disease fibroblasts. GAPDH has also been identified as a potential target for nitric oxide (NO)-mediated cellular toxicity.

特異性

It has been reported that this antibody does not react with GAPDH from E. coli.

應用

This Anti-GAPDH Antibody, clone 6C5, Alexa Fluor^™ 488 conjugate is validated for use in Immunocytochemistry for the detection of GAPDH.

品質

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected GAPDH in A431 cells.

標靶描述

~38 kDa observed

外觀

Purified mouse conjugate antibody in buffer containing PBS 15 mg/ml BSA and 0.1% Sodium Azide.

其他說明

Concentration: Please refer to lot specific datasheet.

法律資訊

ALEXA FLUOR is a trademark of Life Technologies

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable