推薦產品
產品名稱
Anti-Amyloid Precursor Protein Antibody, A4(695), clone 1.D5, clone 1.D5, Chemicon®, from mouse
生物源
mouse
品質等級
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
1.D5, monoclonal
物種活性
human, rat
製造商／商標名
Chemicon®
技術
western blot: suitable
同型
IgG1
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... APP(351)
特異性
Reacts with the natural amyloid precursor protein A4(695).
免疫原
Epitope: A4(695)
Synthetic peptide Alz 90 [amino acids 511-608 of amyloid precursor protein pre A4(695)].
應用
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Neurodegenerative Diseases
This Anti-Amyloid Precursor Protein Antibody, A4(695), clone 1.D5 is validated for use in WB for the detection of Amyloid Precursor Protein.
Western blot: 10-20 μg/mL
Optimal working dilutions must be determined by the end user.
Westen Blot Protocol for MAB349
1) After blotting the SDS-PAGE gel onto nitrocellulose, incubate at room temperature for one hour in 1% PBS in 0.1 mol/L PBS, pH 7.2, to block non-specific background.
2) Incubate the Western blot with MAB349 (10-20 μg/mL) at +4°C overnight shaking well.
3) Wash the Western blots 2 times in PBS-Tween 0.05% for ten minutes. Wash one time in PBS.
4) Incubate the blots with anti-Mouse IgG (Chemicon AP124) for one hour at room temperature.
5) Repeat step #3.
6) Incubate in peroxidase-anti-peroxidase [PAP] (Chemicon PAP14) according to manufacturers directions.
7) Repeat step #3.
8) Incubate in 4-chloro-1-napthol for twenty minutes at room temperature.
9) Repeat step #3.
Optimal working dilutions must be determined by the end user.
Westen Blot Protocol for MAB349
1) After blotting the SDS-PAGE gel onto nitrocellulose, incubate at room temperature for one hour in 1% PBS in 0.1 mol/L PBS, pH 7.2, to block non-specific background.
2) Incubate the Western blot with MAB349 (10-20 μg/mL) at +4°C overnight shaking well.
3) Wash the Western blots 2 times in PBS-Tween 0.05% for ten minutes. Wash one time in PBS.
4) Incubate the blots with anti-Mouse IgG (Chemicon AP124) for one hour at room temperature.
5) Repeat step #3.
6) Incubate in peroxidase-anti-peroxidase [PAP] (Chemicon PAP14) according to manufacturers directions.
7) Repeat step #3.
8) Incubate in 4-chloro-1-napthol for twenty minutes at room temperature.
9) Repeat step #3.
外觀
Format: Purified
Liquid in 0.02M Phosphate buffer, 0.25M NaCl, pH 7.6 with 0.1% sodium azide.
儲存和穩定性
Maintain lyophilized material at +2-8°C for up to 12 months. After reconstitution maintain frozen at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Roles of focal adhesions and fibronectin-mediated cohesion in proliferation of confluent fibroblasts.
Maya Tanaka,Tatsuya Abe,Yoshitaka Hara
Journal of Cellular Physiology null
Axonal injury in young pediatric head trauma: a comparison study of ?-amyloid precursor protein (?-APP) immunohistochemical staining in traumatic and nontraumatic deaths.
Johnson, MW; Stoll, L; Rubio, A; Troncoso, J; Pletnikova, O; Fowler, DR; Li, L
Journal of Forensic Sciences null
Magdolna Pákáski et al.
Neurochemistry international, 47(3), 190-195 (2005-06-16)
Comorbid depression of Alzheimer's disease (AD) is a common mood disorder in the elderly and a broad spectrum of antidepressants have been used for its treatment. Abeta peptides and other derivatives of the amyloid precursor protein (APP) have been implicated
Identification, biogenesis, and localization of precursors of Alzheimer's disease A4 amyloid protein.
Weidemann, A, et al.
Cell, 57, 115-126 (1989)
3,4-Methylenedioxymethamphetamine (MDMA), but not morphine, alters APP processing in the rat brain.
Kalman, Janos, et al.
The International Journal of Neuropsychopharmacology, 10, 183-190 (2007)
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